https://scholars.lib.ntu.edu.tw/handle/123456789/503564
標題: | Anti-HSV activity of digitoxin and its possible mechanisms | 作者: | Su C.-T. Hsu J.T.-A. Hsieh H.-P. Lin P.-H. Chen T.-C. CHUAN-LIANG KAO Lee C.-N. SUI-YUAN CHANG |
公開日期: | 2008 | 卷: | 79 | 期: | 1 | 起(迄)頁: | 62-70 | 來源出版物: | Antiviral Research | 摘要: | Herpes simplex virus type 1 (HSV-1) can establish latent infection in the nervous system and usually leads to life-threatening diseases in immunocompromised individuals upon reactivation. Treatment with conventional nucleoside analogue such as acyclovir is effective in most cases, but drug-resistance may arise due to prolonged treatment in immunocompromised individuals. In this study, we identified an in-use medication, digitoxin, which actively inhibited HSV-1 replication with a 50% effective concentration (EC50) of 0.05 μM. The 50% cytotoxicity concentration (CC50) of digitoxin is 10.66 μM and the derived selective index is 213. Several structural analogues of digitoxin such as digoxin, ouabain octahydrate and G-strophanthin also showed anti-HSV activity. The inhibitory effects of digitoxin are likely to be introduced at the early stage of HSV-1 replication and the virus release stage. The observation that digitoxin can inhibit acyclovir-resistant viruses further implicates that digitoxin represents a novel drug class with distinct antiviral mechanisms from traditional drugs. ? 2008 Elsevier B.V. All rights reserved. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/503564 | ISSN: | 0166-3542 | DOI: | 10.1016/j.antiviral.2008.01.156 | SDG/關鍵字: | aciclovir; digitoxin; digoxin; ouabain; animal cell; antiviral activity; article; controlled study; cytotoxicity; drug inhibition; drug mechanism; Herpes simplex virus 1; immunocompromised patient; nonhuman; priority journal; virus reactivation; virus replication; Animals; Antiviral Agents; Cercopithecus aethiops; Digitoxin; DNA, Viral; Drug Evaluation, Preclinical; Gene Expression; Herpes Simplex; Herpesvirus 1, Human; Humans; Vero Cells; Viral Proteins; Virus Internalization; Virus Replication; Virus Shedding; Human herpesvirus 1; Simplexvirus; Strophanthus gratus |
顯示於: | 醫學檢驗暨生物技術學系 |
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