https://scholars.lib.ntu.edu.tw/handle/123456789/503632
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Yang C.-C. | en_US |
dc.contributor.author | Yang S.-Y. | en_US |
dc.contributor.author | Ho C.-S. | en_US |
dc.contributor.author | Chang J.-F. | en_US |
dc.contributor.author | Liu B.-H. | en_US |
dc.contributor.author | KAI-WEN HUANG | en_US |
dc.creator | Yang C.-C.;Yang S.-Y.;Ho C.-S.;Chang J.-F.;Liu B.-H.;Kai-Wen Huang | - |
dc.date.accessioned | 2020-06-19T02:27:09Z | - |
dc.date.available | 2020-06-19T02:27:09Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 1477-3155 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84988603131&doi=10.1186%2fs12951-014-0044-6&partnerID=40&md5=6c80eed4ecdac9c62dacdae1fa569831 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/503632 | - |
dc.description.abstract | Background: Magnetic nanoparticles functionalized antibodies are used for in-vitro assays on bio-markers. This work demonstrates the synthesis of high-quality magnetic nanoparticles coated with antibodies against carcinoembryonic antigen (CEA). Various characterizations, such as particle size, particle suspension, bio-activity and the stability of bio-magnetic nanoparticles suspended in liquid, are studied. The properties for the assay of CEA molecules in serum are also studied. The assay method used is so-called immunomagnetic reduction. Results: The results show that the effects of common materials in serum that interfere with detected signals are not significant. The low-detection limit is 0.21 ng/ml, which is well below the clinical threshold of 2.5 ng/ml. Conclusions: The dynamic range for the assay of CEA molecules in serum is 500 ng/ml. By assaying serum CEA molecules from 24 normal controls and 30 colorectal-cancer patients, the threshold for the serum-CEA concentration to diagnose colorectal cancer is 4.05 ng/ml, which results in a clinical sensitivity of 0.90 and specificity of 0.87. ? 2014 Yang et al. | - |
dc.publisher | BioMed Central Ltd. | - |
dc.relation.ispartof | Journal of Nanobiotechnology | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | Antibodies; Antigens; Assays; Bioassay; Body fluids; Diseases; Molecules; Nanomagnetics; Particle size; Suspensions (fluids); Synthesis (chemical); Carcinoembryonic antigen; Colorectal cancer; Common materials; Feasibility studies; Low detection limit; Magnetic nano-particles; Normal controls; Particle suspensions; Nanoparticles; antibody; carcinoembryonic antigen; magnetic nanoparticle; nanocoating; nanomaterial; antibody; carcinoembryonic antigen; magnetite nanoparticle; tumor marker; Article; biological activity; biomagnetism; cancer patient; clinical article; colorectal cancer; controlled study; feasibility study; human; immunoassay; limit of detection; liquid; molecular dynamics; molecular stability; particle size; sensitivity and specificity; signal detection; suspension; synthesis; blood; case control study; chemistry; colorectal tumor; immunoassay; immunology; procedures; temperature; Antibodies; Biomarkers, Tumor; Carcinoembryonic Antigen; Case-Control Studies; Colorectal Neoplasms; Humans; Immunoassay; Limit of Detection; Magnetite Nanoparticles; Particle Size; Temperature | - |
dc.title | Development of antibody functionalized magnetic nanoparticles for the immunoassay of carcinoembryonic antigen: A feasibility study for clinical use | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1186/s12951-014-0044-6 | - |
dc.identifier.pmid | 25424410 | - |
dc.identifier.scopus | 2-s2.0-84988603131 | - |
dc.relation.pages | 44 | - |
dc.relation.journalvolume | 12 | - |
dc.relation.journalissue | 1 | - |
item.cerifentitytype | Publications | - |
item.openairetype | journal article | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
crisitem.author.dept | Surgery-NTUH | - |
crisitem.author.dept | Surgery | - |
crisitem.author.dept | Clinical Medicine | - |
crisitem.author.orcid | 0000-0001-6375-8714 | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 醫學系 |
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