https://scholars.lib.ntu.edu.tw/handle/123456789/503646
標題: | Feasibility studies for assaying alpha-fetoprotein using antibody-activated magnetic nanoparticles | 作者: | KAI-WEN HUANG Yang S.-Y. Hong Y.-W. Chieh J.-J. Yang C.-C. Horng H.-E. CHAU-CHUNG WU Hong C.-Y. Yang H.-C. |
公開日期: | 2012 | 卷: | 7 | 起(迄)頁: | 1991-1996 | 來源出版物: | International Journal of Nanomedicine | 摘要: | Some previous reports have already shown the characterizations of immunomagnetic reduction (IMR). The assay technology involves the utilities of biofunctionalized magnetic nanoparticles to label target biomolecules. However, the detection threshold and interference tests for IMR have not been investigated in detail. In this study, alpha-fetoprotein (AFP) was used as a target biomolecule. The signals for AFP solutions of various concentrations, or with interfering materials, were detected via IMR. These samples were also used for characterizing the detection threshold and interference with enzyme-linked immunosorbent assay (ELISA). The results of assaying AFP level with IMR and ELISA were compared. The detection threshold for assaying AFP with IMR was found to be 3 ng/mL, which is 15 times lower than that of ELISA, and definitely suppresses false negative. For the interfering materials noted commonly in serum such as hemoglobin, bilirubin, triglyceride, and vascular endothelial growth factor, there was no detectable interfering effect when assaying AFP with IMR. Several serum samples from normal people and liver-tumor-bearing patients were used for the detections of AFP concentration via IMR. These results reveal the feasibilities of assaying AFP in blood using IMR, as well as achieving high-sensitive and high-specific assay for AFP. ? 2012 Huang et al, publisher and licensee Dove Medical Press Ltd. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84866747613&doi=10.2147%2fIJN.S28245&partnerID=40&md5=25f81b189fee84fe03f10ec5a56c9793 https://scholars.lib.ntu.edu.tw/handle/123456789/503646 |
ISSN: | 1176-9114 | DOI: | 10.2147/IJN.S28245 | SDG/關鍵字: | alpha fetoprotein; bilirubin; hemoglobin; magnetic nanoparticle; triacylglycerol; vasculotropin; AFP protein, human; alpha fetoprotein; immobilized antibody; magnetite nanoparticle; article; bilirubin blood level; cancer diagnosis; comparative effectiveness; controlled study; diagnostic test accuracy study; disease marker; enzyme linked immunosorbent assay; false negative result; feasibility study; hemoglobin blood level; human; immunoassay; liver cell carcinoma; protein blood level; sensitivity and specificity; triacylglycerol blood level; blood; blood analysis; chemistry; comparative study; immunology; limit of detection; liver tumor; methodology; nanomedicine; reference value; alpha-Fetoproteins; Antibodies, Immobilized; Blood Chemical Analysis; Carcinoma, Hepatocellular; Enzyme-Linked Immunosorbent Assay; Feasibility Studies; Humans; Limit of Detection; Liver Neoplasms; Magnetite Nanoparticles; Nanomedicine; Reference Values |
顯示於: | 醫學系 |
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