https://scholars.lib.ntu.edu.tw/handle/123456789/503697
標題: | Molecular subtyping of human T-lymphotropic virus type I (HTLV-I) by a nested polymerase chain reaction-restriction fragment length polymorphism analysis of the envelope gene: Two distinct lineages of HTLV-I in Taiwan | 作者: | YA-CHIEN YANG Hsu T.-Y. Liu M.-Y. Lin M.-T. Chen J.-Y. Yang C.-S. |
關鍵字: | env gene; HTLV-I subtypes; subtype-specific nucleotides | 公開日期: | 1997 | 卷: | 51 | 期: | 1 | 起(迄)頁: | 25-31 | 來源出版物: | Journal of Medical Virology | 摘要: | The major type of human T-lymphotropic virus type I (HTLV-I), generally referred to as the cosmopolitan type, has been grouped into three subtypes (A, B, and C) by phylogenetic analysis of the long terminal repeat sequences of the viral genome. Twelve subtype-specific nucleotide variations have been deduced by comparison between the envelope (env) sequences of 16 HTLV-I strains with defined subtypes and 9 Taiwanese HTLV-I strains. To gain further insights into the molecular epidemiology of HTLV-I and the origin of this virus in Taiwan, a rapid method of identification for the cosmopolitan subtypes was developed by using a nested polymerase chain reaction (PCR) and subsequent restriction fragment length polymorphism (RFLP) studies using the two subtype B specific and four subtype C-specific nucleotides located within the positions 5708 to 6320 of the envgene. The nested PCR-RFLP method was used to subtype HTLV-I from four virus-positive cell lines derived from 1 Japanese and 3 North American patients, as well as 41 blood-unrelated Taiwan Chinese. The sequences of PCR products were determined and the six positions of subtype-specific nucleotide variations were examined. The sequence data completely supported the subtyping data via the nested PCR-RFLP method. The results demonstrated that, as is the case in Japan, at least two distinct cosmopolitan subtypes (A and B) of HTLV-I were present in Taiwan, but the more prevalent subtype in Taiwan is A in contrast to subtype B in Japan. Furthermore, rapid subtyping could facilitate molecular epidemiological studies of HTLV-I infection and linkage between HTLV-I subtypes and virus-associated diseases. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/503697 | ISSN: | 0146-6615 | DOI: | 10.1002/(SICI)1096-9071(199701)51:1<25 | SDG/關鍵字: | article; clinical article; controlled study; dna sequence; genetic polymorphism; human; human t cell leukemia virus 1; phylogeny; polymerase chain reaction; restriction fragment length polymorphism; taiwan; virus strain; Base Sequence; DNA, Viral; Epidemiology, Molecular; Genes, env; HTLV-I Infections; Human T-lymphotropic virus 1; Humans; Molecular Sequence Data; Phylogeny; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Sequence Alignment; Sequence Analysis, DNA; Taiwan |
顯示於: | 醫學檢驗暨生物技術學系 |
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