https://scholars.lib.ntu.edu.tw/handle/123456789/503702
標題: | Establishment and characterization of an HTLV-I cell line from a Taiwanese patient with HTLV-I-associated myelopathy | 作者: | YA-CHIEN YANG Hung T.-p. Wang C.-H. Lin M.-T. Hsu T.-Y. Chen J.-Y. Chen Y.-C. Yang C.-S. |
公開日期: | 1993 | 卷: | 120 | 期: | 1 | 起(迄)頁: | 46-53 | 來源出版物: | Journal of the Neurological Sciences | 摘要: | We describe a Taiwanese woman with chronic progressive myelopathy, in whom Western blot analysis of the serum and cerebrospinal fluid (CSF) displayed positive reactions to human T-lymphotropic virus type I (HTLV-I) proteins, p19, p24, p28, p36, gp46 and p53. HTLV-I proviral genomes were detected in the peripheral blood mononuclear cells (PBMC) and CSF cells by nested polymerase chain reaction and Southern blot hybridization. HTLV-I was successfully isolated from PBMC stimulated with interleukin-2 (IL-2). The established cell line, named THAM-1, was an IL-2-independent T-cell line with CD2+, CD3+, CD4+, CD25+ and HLA-DR+. Retrovirus particles with type C morphology were observed in the THAM-1 cells by electron microscopy, and HTLV-I-related antigens were also demonstrated by immunocytochemical staining and Western blot assay. Southern blot analysis revealed that HTLV-I proviral genomes were integrated into the THAM-1 cellular DNA. In Northern blot analysis, two extra-species of RNA were detected in addition to three typical viral transcripts. For the first time, an HTLV-I-producing T cell line was established from a patient with HTLV-I-associated myelopathy in Taiwan, an HTLV-I non-endemic area. ? 1993. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/503702 | ISSN: | 0022-510X | DOI: | 10.1016/0022-510X(93)90023-R | SDG/關鍵字: | virus protein; adult; article; case report; cell culture; cerebrospinal fluid; etiology; female; human; human cell; human t cell leukemia virus; immunoblotting; mononuclear cell; priority journal; southern blotting; spinal cord disease; ultrastructure; Adult; Blotting, Western; Carrier State; Case Report; Cell Line; Deltaretrovirus Antigens; Female; Genome, Viral; HTLV-I Antibodies; Human; Human T-lymphotropic virus 1; Immunohistochemistry; Male; Microscopy, Electron; Middle Age; Paraparesis, Tropical Spastic; Support, Non-U.S. Gov't; T-Lymphocytes; Tissue Culture; Virion; Virus Integration |
顯示於: | 醫學檢驗暨生物技術學系 |
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