https://scholars.lib.ntu.edu.tw/handle/123456789/504742
Title: | Modeling of cancer metastasis and drug resistance via biomimetic nano-cilia and microfluidics | Authors: | Kuo, C.-T. Chiang, C.-L. Chang, C.-H. Liu, H.-K. Huang, G.-S. RUBY YUN-JU HUANG Lee, H. CHIUN-SHENG HUANG HSIN-YU LEE ANDREW WO |
Issue Date: | 2014 | Journal Volume: | 35 | Journal Issue: | 5 | Start page/Pages: | 1562-1571 | Source: | Biomaterials | Abstract: | Three-dimensional (3D) tissue culture platforms that are capable of mimicking invivo microenvironments to replicate physiological conditions are vital tools in a wide range of cellular and clinical studies. Here, learning from the nature of cilia in lungs - clearing mucus and pathogens from the airway - we develop a 3D culture approach via flexible and kinetic copolymer-based chains (nano-cilia) for diminishing cell-to-substrate adhesion. Multicellular spheroids or colonies were tested for 3-7 days in a microenvironment consisting of generated cells with properties of putative cancer stem cells (CSCs). The dynamic and reversible regulation of epithelial-mesenchymal transition (EMT) was examined in spheroids passaged and cultured in copolymer-coated dishes. The expression of CSC markers, including CD44, CD133, and ABCG2, and hypoxia signature, HIF-1α, was significantly upregulated compared to that without the nano-cilia. In addition, these spheroids exhibited chemotherapeutic resistance invitro and acquired enhanced metastatic propensity, as verified from microfluidic chemotaxis assay designed to replicate invivo-like metastasis. The biomimetic nano-cilia approach and microfluidic device may offer new opportunities to establish a rapid and cost-effective platform for the study of anti-cancer therapeutics and CSCs. ? 2013 Elsevier Ltd. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84890148410&doi=10.1016%2fj.biomaterials.2013.11.008&partnerID=40&md5=370cd8a4a8cf5df9a3a537fce5deb454 https://scholars.lib.ntu.edu.tw/handle/123456789/504742 |
ISSN: | 0142-9612 | DOI: | 10.1016/j.biomaterials.2013.11.008 | SDG/Keyword: | Anti-cancer therapeutics; CSC; Drug resistance; EMT; Epithelial-mesenchymal transition; Metastasis; Multicellular spheroid; Physiological condition; Biochemistry; Biomimetics; Copolymers; Diseases; Pathology; Stem cells; Three dimensional; Tissue culture; Microfluidics; biomimetic material; breast cancer resistance protein; CD133 antigen; copolymer; Hermes antigen; hypoxia inducible factor 1alpha; nanocilium; nanomaterial; unclassified drug; article; cancer chemotherapy; cancer resistance; cancer stem cell; cell adhesion; cell assay; cell culture; cell line; chemotaxis; controlled study; cost effectiveness analysis; epithelial mesenchymal transition; eukaryotic flagellum; female; human; human cell; immunofluorescence; in vitro study; learning; metastasis; microfluidics; mucus secretion; multicellular spheroid; ovary cancer; phenotype; priority journal; three dimensional imaging; tissue culture; tumor microenvironment; upregulation; Biomimetic; CSC; Drug resistance; EMT; Metastasis; Microfluidics; Biomimetics; Cell Line, Tumor; Cilia; Drug Resistance, Neoplasm; Female; Fluorescent Antibody Technique; Humans; Microfluidics; Models, Biological; Nanostructures; Neoplasm Metastasis; Neoplasms |
Appears in Collections: | 醫學系 |
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