https://scholars.lib.ntu.edu.tw/handle/123456789/504742
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Kuo, C.-T. | en_US |
dc.contributor.author | Chiang, C.-L. | en_US |
dc.contributor.author | Chang, C.-H. | en_US |
dc.contributor.author | Liu, H.-K. | en_US |
dc.contributor.author | Huang, G.-S. | en_US |
dc.contributor.author | RUBY YUN-JU HUANG | en_US |
dc.contributor.author | Lee, H. | en_US |
dc.contributor.author | CHIUN-SHENG HUANG | en_US |
dc.contributor.author | HSIN-YU LEE | en_US |
dc.contributor.author | ANDREW WO | en_US |
dc.creator | Wo A.M.;Huang C.-S.;Lee H.;RUBY YUN-JU HUANG;Huang G.-S.;Liu H.-K.;Chang C.-H.;Chiang C.-L.;Kuo C.-T. | - |
dc.date.accessioned | 2020-06-26T01:17:02Z | - |
dc.date.available | 2020-06-26T01:17:02Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84890148410&doi=10.1016%2fj.biomaterials.2013.11.008&partnerID=40&md5=370cd8a4a8cf5df9a3a537fce5deb454 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/504742 | - |
dc.description.abstract | Three-dimensional (3D) tissue culture platforms that are capable of mimicking invivo microenvironments to replicate physiological conditions are vital tools in a wide range of cellular and clinical studies. Here, learning from the nature of cilia in lungs - clearing mucus and pathogens from the airway - we develop a 3D culture approach via flexible and kinetic copolymer-based chains (nano-cilia) for diminishing cell-to-substrate adhesion. Multicellular spheroids or colonies were tested for 3-7 days in a microenvironment consisting of generated cells with properties of putative cancer stem cells (CSCs). The dynamic and reversible regulation of epithelial-mesenchymal transition (EMT) was examined in spheroids passaged and cultured in copolymer-coated dishes. The expression of CSC markers, including CD44, CD133, and ABCG2, and hypoxia signature, HIF-1α, was significantly upregulated compared to that without the nano-cilia. In addition, these spheroids exhibited chemotherapeutic resistance invitro and acquired enhanced metastatic propensity, as verified from microfluidic chemotaxis assay designed to replicate invivo-like metastasis. The biomimetic nano-cilia approach and microfluidic device may offer new opportunities to establish a rapid and cost-effective platform for the study of anti-cancer therapeutics and CSCs. ? 2013 Elsevier Ltd. | - |
dc.relation.ispartof | Biomaterials | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | Anti-cancer therapeutics; CSC; Drug resistance; EMT; Epithelial-mesenchymal transition; Metastasis; Multicellular spheroid; Physiological condition; Biochemistry; Biomimetics; Copolymers; Diseases; Pathology; Stem cells; Three dimensional; Tissue culture; Microfluidics; biomimetic material; breast cancer resistance protein; CD133 antigen; copolymer; Hermes antigen; hypoxia inducible factor 1alpha; nanocilium; nanomaterial; unclassified drug; article; cancer chemotherapy; cancer resistance; cancer stem cell; cell adhesion; cell assay; cell culture; cell line; chemotaxis; controlled study; cost effectiveness analysis; epithelial mesenchymal transition; eukaryotic flagellum; female; human; human cell; immunofluorescence; in vitro study; learning; metastasis; microfluidics; mucus secretion; multicellular spheroid; ovary cancer; phenotype; priority journal; three dimensional imaging; tissue culture; tumor microenvironment; upregulation; Biomimetic; CSC; Drug resistance; EMT; Metastasis; Microfluidics; Biomimetics; Cell Line, Tumor; Cilia; Drug Resistance, Neoplasm; Female; Fluorescent Antibody Technique; Humans; Microfluidics; Models, Biological; Nanostructures; Neoplasm Metastasis; Neoplasms | - |
dc.title | Modeling of cancer metastasis and drug resistance via biomimetic nano-cilia and microfluidics | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.biomaterials.2013.11.008 | - |
dc.identifier.pmid | 24269156 | - |
dc.identifier.scopus | 2-s2.0-84890148410 | - |
dc.relation.pages | 1562-1571 | - |
dc.relation.journalvolume | 35 | - |
dc.relation.journalissue | 5 | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
crisitem.author.dept | School of Medicine | - |
crisitem.author.dept | Master's Program in Smart Medicine and Health Informatics (SMARTMHI) | - |
crisitem.author.dept | Surgery-NTUH | - |
crisitem.author.dept | Surgery | - |
crisitem.author.dept | Biomedical Electronics and Bioinformatics | - |
crisitem.author.dept | Life Science | - |
crisitem.author.dept | Center for Biotechnology | - |
crisitem.author.dept | Electrical Engineering | - |
crisitem.author.dept | Applied Mechanics | - |
crisitem.author.orcid | 0000-0001-6376-3185 | - |
crisitem.author.orcid | 0000-0002-6557-211X | - |
crisitem.author.orcid | 0000-0002-1477-0183 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | International College | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Electrical Engineering and Computer Science | - |
crisitem.author.parentorg | College of Life Science | - |
crisitem.author.parentorg | Others: University-Level Research Centers | - |
crisitem.author.parentorg | College of Electrical Engineering and Computer Science | - |
crisitem.author.parentorg | College of Engineering | - |
顯示於: | 醫學系 |
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