https://scholars.lib.ntu.edu.tw/handle/123456789/504752
標題: | Configurable 2D and 3D spheroid tissue cultures on bioengineered surfaces with acquisition of epithelial-mesenchymal transition characteristics | 作者: | HSIN-YU LEE Chiang, C.-L. RUBY YUN-JU HUANG Lee, H. ANDREW WO |
公開日期: | 2012 | 卷: | 4 | 期: | 9 | 起(迄)頁: | e27 | 來源出版物: | NPG Asia Materials | 摘要: | Both two-dimensional (2D) and three-dimensional (3D) biomaterial-based culture platforms that are capable of mimicking the in vivo microenvironment to recapitulate the physiological conditions are vital tools in a wide range of cellular and clinical research. Here we report tissue cultures in a microfluidic chip that allows deterministic patterning of cells in 2D/3D. The chip contains a cell-supporting membrane bioengineered to attain either 2D or 3D cell patterns by selectable deposition of extracellular matrix molecules. Results show a cell-trapping rate as high as 97% in our microchip. Tuning of the surface enables not only highly controlled geometry of the monolayer (2D) cell mass but also 3D culture of uniformly sized multicellular spheroids. The 3D spheroid culture of human epithelial ovarian cancer cells in the microfluidic chip resulted in acquisition of mesenchymal traits-increased expressions of N-cadherin, vimentin and fibronectin-and lowered expression of epithelial marker (CD326/epithelial cell adhesion molecule) compared with that in traditional 2D cultures, which is indicative of epithelial-mesenchymal transition in the spheres. In conclusion, these results offer new opportunities to achieve active control of 2D cellular patterns and 3D multicellular spheroids on demand, and may be amenable toward the study of the metastatic processes by in vitro modeling. ? 2012 Nature Japan K.K. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84870899981&doi=10.1038%2fam.2012.50&partnerID=40&md5=a0ec1bd16ee7e0a21a2416c74de2f756 https://scholars.lib.ntu.edu.tw/handle/123456789/504752 |
ISSN: | 1884-4049 | DOI: | 10.1038/am.2012.50 | SDG/關鍵字: | Cell adhesion molecules; Cell patterns; EMT; Epithelial-mesenchymal transition; Extracellular matrix molecules; Multicellular spheroid; Physiological condition; Spheroid; Biological materials; Enzyme inhibition; Fluidic devices; Microfluidics; Microsystems; Monolayers; Three dimensional; Three dimensional computer graphics; Tissue culture; Two dimensional |
顯示於: | 醫學系 |
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