https://scholars.lib.ntu.edu.tw/handle/123456789/506291
標題: | Inspiratory muscle training attenuates irradiation-induced diaphragm dysfunction | 作者: | LI-YING WANG Yang P.-Y. Chen Y.-J. Wu H.-D. Huang Y.-H. Hsieh C.-H. |
公開日期: | 2019 | 出版社: | E-Century Publishing Corporation | 卷: | 11 | 期: | 9 | 起(迄)頁: | 5599-5610 | 來源出版物: | American Journal of Translational Research | 摘要: | Because radiotherapy (RT) can induce diaphragm dysfunction, this study investigated the protective effect of inspiratory muscle training (IMT) on RT-induced diaphragm damage in patients with esophageal cancer during concurrent chemoradiotherapy (CCRT) in a preclinical setting, and an animal model was designed to confirm and explore the underlying mechanism. Six subjects who underwent CCRT were randomly enrolled in the control or concurrent-IMT group (n=3 per group). The training intensity was set to 30% maximal effort. The diaphragmatic function and functional exercise capacity were assessed weekly during the course of CCRT. Furthermore, Sprague-Dawley (SD) rats were randomly assigned to receive IMT using the tracheal banding method over a 1-week period (n=6) or the sham group (n=6). After training was completed, 5-Gy RT was applied to the diaphragm. All the rats were sacrificed 24 h following RT, and their diaphragms were removed and examined for contractile function, antioxidant capacity, and oxidative injury. In patients receiving IMT, the diaphragm activation efficiency and fatigability and the functional exercise capacity were improved during the CCRT course. The animals belonging to the training group demonstrated significantly higher peak twitch (P<0.01) and tetanus tension (P<0.001), less fatigue (P=0.04), lower protein carbonyl levels (P<0.01) and higher Cu/Zn-SOD and Mn-SOD mRNA expression levels (both P<0.05) compared with those belonging to the control group. Preclinical human and animal models show that the IMT-conditioned diaphragm exhibits better resistance to off-target irradiation damage, but studies with a larger patient sample size are warranted to confirm the applicability of this concept in clinical practice. ? 2019 E-Century Publishing Corporation. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85076981127&partnerID=40&md5=2d5da2ef616b5db4091c100f4c0e2eb0 https://scholars.lib.ntu.edu.tw/handle/123456789/506291 |
ISSN: | 1943-8141 | SDG/關鍵字: | atropine; carbonyl derivative; copper zinc superoxide dismutase; manganese superoxide dismutase; tiletamine plus zolazepam; xylazine; animal experiment; animal model; animal tissue; antioxidant activity; Article; chemoradiotherapy; diaphragm disease; diaphragm strip contractility; diaphragmatic function; electromyography; esophagus cancer; functional exercise capacity; inspiratory muscle training; irradiation; lung function test; maximum static inspiratory pressure; mRNA expression level; muscle training; musculoskeletal system parameters; nonhuman; oxidative injury; physical parameters; protein expression; rat; real time polymerase chain reaction; reverse transcription polymerase chain reaction; spirometry; total lung capacity; Western blotting |
顯示於: | 物理治療學系所 |
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