https://scholars.lib.ntu.edu.tw/handle/123456789/506298
標題: | Diaphragm contractile dysfunction causes by off-target low-dose irradiation | 作者: | Hsieh C.-H. Lin Y.-C. Chen Y.-J. Wu H.-D. LI-YING WANG |
公開日期: | 2016 | 出版社: | E-Century Publishing Corporation | 卷: | 8 | 期: | 3 | 起(迄)頁: | 1510-1517 | 來源出版物: | American Journal of Translational Research | 摘要: | Background: Diaphragm is a primary inspiratory muscle and often receives off-target dose in patients with thoracic radiotherapy, and whether acute effect of low dose irradiation would cause contractile dysfunction of the diaphragm remains unclear. We use a rat model to investigate the effect of low-dose irradiation on diaphragm contractile function in the current study. Methods: The radiation dose distributions in patients with esophageal cancer receiving radiotherapy were calculated to determine the dose received by the off-target diaphragm area. Rats were randomly assigned to an irradiated or a non-irradiated control group (n = 10 per group). A single-fraction of 5 Gy radiation was then delivered to the diaphragms of Sprague-Dawley rats in the irradiated group. The control group received sham irradiation (0 Gy). Rats were sacrificed 24 hours after the irradiation procedures and diaphragms were removed en bloc for contractile function assessment, oxidative injury and DNA damage analysis. Oxidative injury was determined by analyzing concentration of protein carbonyls and DNA damage was determined by analyzing retention of γH2AX foci in nuclei of diaphragmatic tissue. Results: At 24 hours after delivery of a single dose of 5 Gy radiation, specific twitch (p = 0.03) and tetanus tension (p = 0.02) were significantly lower in the irradiated group than in the control group. The relative force-frequency curves showed a significant downward shift in the irradiated group. Protein carbonyl level (p < 0.01) and percentage of γH2AX-positive diaphragm muscle cells were significantly higher in the irradiated group than in the control group 24 hours after irradiation (58% vs. 30%, p = 0.01). Conclusions: Off-target low dose irradiation could induce acute contractile dysfunction of the diaphragm which was related to radiation-induced direct DNA and indirect oxidative damage. ? 2016, E-Century Publishing Corporation. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84964424956&partnerID=40&md5=ae56c134ffe518ba389ec13c73b48bed https://scholars.lib.ntu.edu.tw/handle/123456789/506298 |
ISSN: | 1943-8141 | SDG/關鍵字: | carbonyl derivative; histone H2AX; animal experiment; animal model; animal tissue; Article; cancer radiotherapy; controlled study; diaphragm contractile dysfunction; diaphragm disease; DNA damage; heart contraction; immunohistochemistry; male; nonhuman; oxidative stress; radiation dose; rat |
顯示於: | 物理治療學系所 |
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