|Title:||Ganoderic acid X, a lanostanoid triterpene, inhibits topoisomerases and induces apoptosis of cancer cells||Authors:||Li C.-H.
|Issue Date:||2005||Journal Volume:||77||Journal Issue:||3||Start page/Pages:||252-265||Source:||Life Sciences||Abstract:||
Lanostanoid triterpenes isolated from Ganoderma amboinense were found to inhibit the growth of numerous cancer cell lines, and some of them inhibited the activities of topoisomerases I and IIα in vitro. Among the bioactive isolates, one of the most potent triterpene was identified to be 3α-hydroxy-15α-acetoxy-lanosta-7,9(11),24-trien-26-oic acid, ganoderic acid X (GAX). Treatment of human hepatoma HuH-7 cells with GAX caused immediate inhibition of DNA synthesis as well as activation of ERK and JNK mitogen-activated protein kinases, and cell apoptosis. Molecular events of apoptosis including degradation of chromosomal DNA, decrease in the level of Bcl-xL, the disruption of mitochondrial membrane, cytosolic release of cytochrome c and activation of caspase-3 were elucidated. The ability of GAX to inhibit topoisomerases and to sensitize the cancer cells toward apoptosis fulfills the feature of a potential anticancer drug. ? 2005 Elsevier Inc. All rights reserved.
|URI:||https://scholars.lib.ntu.edu.tw/handle/123456789/507693||ISSN:||0024-3205||DOI:||10.1016/j.lfs.2004.09.045||metadata.dc.subject.other:||3alpha hydroxy 15alpha acetoxylanosta 7,9(11),24 trien 26 oic acid; caspase 3; cytochrome c; DNA topoisomerase; DNA topoisomerase (ATP hydrolysing); ganoderma amboinense extract; mitogen activated protein kinase; plant extract; protein bcl xl; stress activated protein kinase; topoisomerase IIalpha; triterpene; unclassified drug; apoptosis; article; cancer cell culture; cancer inhibition; chromosome; controlled study; cytosol; DNA degradation; DNA synthesis inhibition; drug identification; drug isolation; drug potency; drug sensitization; enzyme activation; enzyme activity; enzyme inhibition; enzyme release; Ganoderma; ganoderma amboinense; hepatoma cell; human; human cell; medicinal plant; membrane damage; mitochondrial membrane; molecule; Antigens, Neoplasm; Antineoplastic Agents; Apoptosis; Caspase 3; Caspases; Cell Line, Tumor; Cell Proliferation; DNA Topoisomerases, Type I; DNA Topoisomerases, Type II, Eukaryotic; DNA-Binding Proteins; Enzyme Activation; Extracellular Signal-Regulated MAP Kinases; Ganoderma; Humans; JNK Mitogen-Activated Protein Kinases; Lanosterol; Medicine, Chinese Traditional; Molecular Structure; Neoplasms; Triterpenes; Ganoderma
|Appears in Collections:||醫學檢驗暨生物技術學系|
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