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  1. NTU Scholars
  2. 醫學院
  3. 生理學科所
Please use this identifier to cite or link to this item: https://scholars.lib.ntu.edu.tw/handle/123456789/507735
Title: ADAM9 enhances CDCP1 protein expression by suppressing MIR-218 for lung tumor metastasis
Authors: Chiu K.-L.
Kuo T.-T.
Kuok Q.-Y.
Lin Y.-S.
Hua C.-H.
Lin C.-Y.
Su P.-Y.
LIANG-CHUAN LAI 
Sher Y.-P.
Issue Date: 2015
Publisher: Nature Publishing Group
Journal Volume: 5
Start page/Pages: 16426
Source: Scientific Reports
Abstract: 
Metastasis is the leading cause of death in cancer patients due to the difficulty of controlling this complex process. MicroRNAs (miRNA), endogenous noncoding short RNAs with important biological and pathological functions, may play a regulatory role during cancer metastasis, but this role has yet to be fully defined. We previously demonstrated that ADAM9 enhanced the expression of the pro-migratory protein CDCP1 to promote lung metastasis; however, the regulatory process remains unknown. Here we demonstrate that endogenous miR-218, which is abundant in normal lung tissue but suppressed in lung tumors, is regulated during the process of ADAM9-mediated CDCP1 expression. Suppression of miR-218 was associated with high migration ability in lung cancer cells. Direct interaction between miR-218 and the 3′-UTR of CDCP1 mRNAs was detected in luciferase-based transcription reporter assays. CDCP1 protein levels decreased as expression levels of miR-218 increased, and increased in cells treated with miR-218 antagomirs. Induction of miR-218 inhibited tumor cell mobility, anchorage-free survival, and tumor-initiating cell formation in vitro and delayed tumor metastases in mice. Our findings revealed an integrative tumor suppressor function of miR-218 in lung carcinogenesis and metastasis.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-84946866079&doi=10.1038%2fsrep16426&partnerID=40&md5=6e45f6aba28c00fa3ee6232743c4858a
https://scholars.lib.ntu.edu.tw/handle/123456789/507735
ISSN: 2045-2322
DOI: 10.1038/srep16426
metadata.dc.subject.other: ADAM protein; ADAM9 protein, human; CDCP1 protein, human; cell adhesion molecule; leukocyte antigen; membrane protein; messenger RNA; microRNA; MIRN218 microRNA, human; small interfering RNA; tumor protein; animal; binding site; cell motion; cell survival; chemistry; disease model; gene expression regulation; genetics; human; lung tumor; metabolism; metastasis; mortality; mouse; nucleotide sequence; pathology; RNA interference; tumor cell line; xenograft; ADAM Proteins; Animals; Antigens, CD; Base Sequence; Binding Sites; Cell Adhesion Molecules; Cell Line, Tumor; Cell Movement; Cell Survival; Disease Models, Animal; Gene Expression Regulation, Neoplastic; Heterografts; Humans; Lung Neoplasms; Membrane Proteins; Mice; MicroRNAs; Neoplasm Metastasis; Neoplasm Proteins; RNA Interference; RNA, Messenger; RNA, Small Interfering
[SDGs]SDG3
Appears in Collections:生理學科所

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臺大位居世界頂尖大學之列,為永久珍藏及向國際展現本校豐碩的研究成果及學術能量,圖書館整合機構典藏(NTUR)與學術庫(AH)不同功能平台,成為臺大學術典藏NTU scholars。期能整合研究能量、促進交流合作、保存學術產出、推廣研究成果。

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