https://scholars.lib.ntu.edu.tw/handle/123456789/508012
標題: | Human TLR3 recognizes dengue virus and modulates viral replication in vitro | 作者: | Tsai Y.-T. SUI-YUAN CHANG Lee C.-N. CHUAN-LIANG KAO |
公開日期: | 2009 | 卷: | 11 | 期: | 4 | 起(迄)頁: | 604-615 | 來源出版物: | Cellular Microbiology | 摘要: | The elicitation of large amount inflammatory cytokine in serum has been developed as the cause of the plasma leakage in dengue fever (DF)/dengue haemorrhagic fever (DHF) infection. Virus recognition in innate immunity is the key. The Toll-like receptors (TLRs) play an important role in pathogen recognition towards cytokine induction among several viruses; however, the role of TLRs on innate immune recognition against DENV remains unclear. This study aims at the interaction between dengue virus (DENV) and human TLRs at the incipient stage of infection in vitro. Our experiment reveals that stably expression of TLR3, 7, 8 on HEK293 enables IL-8 secretion after DENV recognition. By the model of human monocytic cells U937, we demonstrated the trigger of IL-8 after viral recognition of human monocytic cell is primary through TLR3 following endosomal acidification. Silencing of TLR3 in U937 cells significantly blocks the DENV-induced IL-8 production. Besides, the interaction is further corroborated by colocalization of TLR3 and DENV RNA upon DENV internalization. Furthermore, in this study we found the expression of TLR3 can mediate strong IFN-α/β release and inhibit DENV viral replication significantly, thus limit the cytopathic effect. ? 2009 Blackwell Publishing Ltd. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/508012 | ISSN: | 1462-5814 | DOI: | 10.1111/j.1462-5822.2008.01277.x | SDG/關鍵字: | alpha interferon; beta interferon; interleukin 8; toll like receptor 3; virus RNA; article; cell strain HEK293; confocal microscopy; controlled study; cytokine release; cytopathogenic effect; dengue; Dengue virus; enzyme linked immunosorbent assay; flow cytometry; human; human cell; in vitro study; monocyte; nonhuman; priority journal; protein expression; protein interaction; protein localization; real time polymerase chain reaction; RNA isolation; virus entry; virus inhibition; virus replication; Cell Line; Dengue; Dengue Virus; Host-Pathogen Interactions; Humans; Monocytes; Toll-Like Receptor 3; U937 Cells; Virus Replication; Dengue virus |
顯示於: | 醫學檢驗暨生物技術學系 |
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