https://scholars.lib.ntu.edu.tw/handle/123456789/508079
Title: | Analysis of the steps involved in dengue virus entry into host cells | Authors: | Hung S.-L. Lee P.-L. Chen H.-W. Chen L.-K. CHUAN-LIANG KAO King C.-C. |
Issue Date: | 1999 | Journal Volume: | 257 | Journal Issue: | 1 | Start page/Pages: | 156-167 | Source: | Virology | Abstract: | The initial steps of dengue viral entry have been divided into adsorption and penetration using acid glycine treatment to inactivate extracellular virus after attachment to baby hamster kidney (BHK) cells but prior to penetration. First, we showed that virus infection was accomplished within 2 h after adsorption. Second, the assay was used to examine the properties of dengue envelope E protein-specific monoclonal antibodies (MAbs), lectins, and heparin. We found that three MAbs, 17-2, 46-9, and 51-3, may neutralize dengue 2 virus (DEN-2) through inhibition of not only viral attachment but also of penetration. However, one MAb, 56-3.1, interfered specifically with attachment. Therefore, the functional domains of E protein involved in attachment and penetration may be different. Moreover, studies with lectins indicated that carbohydrates, especially a-mannose residues, present on the virion glycoproteins may contribute to binding and penetration of the virus into BHK and mosquito 06/36 cells. Finally, virus infectivity was inhibited by heparin through its blocking effects at both virus attachment and penetration. This suggests that cell surface heparan sulfate functions in both viral attachment and penetration of DEN-2 virus. In conclusion, our results further elucidated some aspects of the dengue virus entry process. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/508079 | ISSN: | 0042-6822 | DOI: | 10.1006/viro.1999.9633 | SDG/Keyword: | carbohydrate; glycoprotein; heparan sulfate; lectin; mannose; monoclonal antibody; protein; adsorption; animal cell; article; cell surface; Dengue virus; Flavivirus; hamster; host cell; mouse; nonhuman; priority journal; shock; virion; virus envelope; virus infection |
Appears in Collections: | 醫學檢驗暨生物技術學系 |
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