https://scholars.lib.ntu.edu.tw/handle/123456789/509454
標題: | A novel virulence gene in Klebsiella pneumoniae strains causing primary liver abscess and septic metastatic complications | 作者: | CHI-TAI FANG Chuang, Yi-Ping CHIA-TUNG SHUN SHAN-CHWEN CHANG JIN-TOWN WANG |
關鍵字: | pathogenesis; serum resistance; virulence; genes; liver abscess; meningitis; endophthalmitis | 公開日期: | 1-三月-2004 | 出版社: | ROCKEFELLER UNIV PRESS | 卷: | 199 | 期: | 5 | 起(迄)頁: | 697 | 來源出版物: | The Journal of experimental medicine | 摘要: | Primary Klebsiella pneumoniae liver abscess complicated with metastatic meningitis or endophthalmitis is a globally emerging infectious disease. Its pathogenic mechanism remains unclear. The bacterial virulence factors were explored by comparing clinical isolates. Differences in mucoviscosity were observed between strains that caused primary liver abscess (invasive) and those that did not (noninvasive). Hypermucoviscosity correlated with a high serum resistance and was more prevalent in invasive strains (52/53 vs. 9/52; P < 0.0001). Transposon mutagenesis identified candidate virulence genes. A novel 1.2-kb locus, magA, which encoded a 43-kD outer membrane protein, was significantly more prevalent in invasive strains (52/53 vs. 14/52; P < 0.0001). The wild-type strain produced a mucoviscous exopolysaccharide web, actively proliferated in nonimmune human serum, resisted phagocytosis, and caused liver microabscess and meningitis in mice. However, magA- mutants lost the exopolysaccharide web and became extremely serum sensitive, phagocytosis susceptible, and avirulent to mice. Virulence was restored by complementation using a magA-containing plasmid. We conclude that magA fits molecular Koch's postulates as a virulence gene. Thus, this locus can be used as a marker for the rapid diagnosis and for tracing the source of this emerging infectious disease. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/509454 | ISSN: | 0022-1007 | DOI: | 10.1084/jem.20030857 | SDG/關鍵字: | bacterium lipopolysaccharide; complement component C3; DNA; exopolysaccharide; outer membrane protein; protein magA; unclassified drug; virulence factor; animal experiment; animal model; article; bacterial gene; bacterial meningitis; bacterial strain; bacterial virulence; controlled study; DNA sequence; endophthalmitis; female; gene locus; inverse polymerase chain reaction; Klebsiella pneumoniae; liver abscess; magA gene; mouse; nonhuman; phagocytosis; plasmid; priority journal; sepsis; transposon; viscosity; Animals; Base Sequence; DNA, Bacterial; Endophthalmitis; Female; Genes, Bacterial; Genetic Complementation Test; Humans; Klebsiella Infections; Klebsiella pneumoniae; Liver Abscess; Meningitis, Bacterial; Mice; Mice, Inbred BALB C; Mutation; Sepsis; Virulence; Viscosity |
顯示於: | 流行病學與預防醫學研究所 |
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