https://scholars.lib.ntu.edu.tw/handle/123456789/510263
標題: | Autophagy in fibroblasts induced by cigarette smoke extract promotes invasion in lung cancer cells | 作者: | HSIN-HAN HOU Pan, Huei-Jyuan WEI-YU LIAO Lee, Chau-Hwang CHONG-JEN YU |
公開日期: | 2020 | 出版社: | Wiley-Liss Inc. | 來源出版物: | International Journal of Cancer | 摘要: | We investigated the effects of cigarette smoke extract (CSE) on lung fibroblasts and found that the invasiveness of lung cancer cells was facilitated by the conditioned medium from CSE-treated fibroblasts. CSE induced autophagy in fibroblasts and increased the expression of autophagy-related proteins, including optineurin and Ras-related protein Rab1B. Afterward, the fibroblasts produced high levels of interleukin-8 (IL-8), which promoted cancer cell invasion. The inhibition of either optineurin or Rab1B abrogated a rise in microtubule-associated protein 1 light chain 3 β and a decrease in p62 protein, as well as the production of IL-8, in CSE-treated fibroblasts. A three-dimensional invasion assay using cancer cell spheroids revealed that the invasion of cancer cells alone and the fibroblast-led cancer cell invasion were both enhanced by the conditioned media from CSE-treated fibroblasts. These results suggest that cigarette smoke may induce autophagy and IL-8 secretion in lung fibroblasts and modify the microenvironment to favor invasion of lung cancer cells. ? 2020 UICC |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85087316263&doi=10.1002%2fijc.33127&partnerID=40&md5=388debf83ae3d36d9519522dba0ff9ec https://scholars.lib.ntu.edu.tw/handle/123456789/510263 |
ISSN: | 0020-7136 | DOI: | 10.1002/ijc.33127 | SDG/關鍵字: | cigarette smoke; guanine nucleotide binding protein; interleukin 8; microtubule associated protein; microtubule associated protein 1 light chain 3beta; optineurin; Rab1B protein; sequestosome 1; unclassified drug; carrier protein; cell cycle protein; CXCL8 protein, human; interleukin 8; OPTN protein, human; Rab protein; Rab1B protein, human; Article; autophagy (cellular); controlled study; cytokine production; cytokine release; human; human cell; lung fibroblast; non small cell lung cancer; priority journal; protein expression; tumor invasion; tumor spheroid; adverse event; autophagy; cancer associated fibroblast; cell motion; conditioned medium; cytology; drug effect; gene knockdown; genetics; immunology; lung; lung tumor; metabolism; multicellular spheroid; non small cell lung cancer; pathology; smoke; smoking; tobacco; tumor cell line; tumor invasion; tumor microenvironment; Autophagy; Cancer-Associated Fibroblasts; Carcinoma, Non-Small-Cell Lung; Cell Cycle Proteins; Cell Line, Tumor; Cell Movement; Culture Media, Conditioned; Gene Knockdown Techniques; Humans; Interleukin-8; Lung; Lung Neoplasms; Membrane Transport Proteins; Neoplasm Invasiveness; rab1 GTP-Binding Proteins; Smoke; Smoking; Spheroids, Cellular; Tobacco; Tumor Microenvironment |
顯示於: | 醫學系 |
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