https://scholars.lib.ntu.edu.tw/handle/123456789/511003
Title: | Imaging Endogenous Bilirubins with Two-Photon Fluorescence of Bilirubin Dimers | Authors: | Shen Y.-F. Tsai M.-R. Chen S.-C. Leung Y.-S. Hsieh C.-T. Chen Y.-S. Huang F.-L. Obena R.P. Zwulueta M.M.L. Huang H.-Y. WEN-JENG LEE Tang K.-C. Kung C.-T. MIN-HUEY CHEN Shieh D.-B. Chen Y.-J. Liu T.-M. Chou P.-T. Sun C.-K. |
Issue Date: | 2015 | Journal Volume: | 87 | Journal Issue: | 15 | Start page/Pages: | 7575-7582 | Source: | Analytical Chemistry | Abstract: | (Graph Presented) On the basis of an infrared femtosecond Cr:forsterite laser, we developed a semiquantitative method to analyze the microscopic distribution of bilirubins. Using 1230 nm femtosecond pulses, we selectively excited the two-photon red fluorescence of bilirubin dimers around 660 nm. Autofluorescences from other endogenous fluorophores were greatly suppressed. Using this distinct fluorescence measure, we found that poorly differentiated hepatocellular carcinoma (HCC) tissues on average showed 3.7 times lower concentration of bilirubins than the corresponding nontumor parts. The corresponding fluorescence lifetime measurements indicated that HCC tissues exhibited a longer lifetime (500 ps) than that of nontumor parts (300 ps). Similarly, oral cancer cell lines had longer lifetimes (>330 ps) than those of nontumor ones (250 ps). We anticipate the developed methods of bilirubin molecular imaging to be useful in diagnosing cancers or studying the dynamics of bilirubin metabolisms in live cells. ? 2015 American Chemical Society. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/511003 | ISSN: | 0003-2700 | DOI: | 10.1021/acs.analchem.5b01903 | SDG/Keyword: | Cell culture; Dimers; Diseases; Electromagnetic pulse; Histology; Molecular imaging; Photons; Tissue; Cr : Forsterite laser; Endogenous fluorophores; Fluorescence lifetime measurements; Hepatocellular carcinoma; Microscopic distribution; Oral cancer cells; Red fluorescence; Two photon fluorescence; Fluorescence; bilirubin; Carcinoma, Hepatocellular; chemistry; dimerization; human; liver; liver tumor; metabolism; molecular diagnosis; Mouth Neoplasms; multiphoton microscopy; pathology; tumor cell line; Bilirubin; Carcinoma, Hepatocellular; Cell Line, Tumor; Dimerization; Humans; Liver; Liver Neoplasms; Microscopy, Fluorescence, Multiphoton; Molecular Diagnostic Techniques; Mouth Neoplasms |
Appears in Collections: | 醫學院附設醫院 (臺大醫院) |
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