https://scholars.lib.ntu.edu.tw/handle/123456789/519459
標題: | Assay of plasma phosphorylated tau protein (threonine 181) and total tau protein in early-stage Alzheimer's disease | 作者: | Yang C.-C. MING-JANG CHIU TA-FU CHEN Chang H.-L. Liu B.-H. Yang S.-Y. |
公開日期: | 2018 | 出版社: | IOS Press | 卷: | 61 | 期: | 4 | 起(迄)頁: | 1323-1332 | 來源出版物: | Journal of Alzheimer's Disease | 摘要: | The feasibility of assaying plasma phosphorylated tau protein (threonine 181), denoted p-tau181, using immunomagnetic reduction (IMR) is explored. The reagent for assaying p-tau181 with IMR was synthesized, and its analytic performances were characterized. Seventy-three subjects were recruited. Each participant was examined with neuropsychological tests, magnetic resonance imaging, and IMR assay for plasma p-tau181. Using commercially available IMR kits, the plasma total tau protein (T-tau) of each subject was assayed. The dynamic range for assaying p-tau181 using IMR was 1.96×10-2 pg/ml to 104 pg/ml. There was no significant interference from total tau protein in the assay of p-tau181. The measured concentrations of plasma p-tau181 were 2.46±1.09 pg/ml for healthy controls, 4.41±1.85 pg/ml for MCI due to AD, and 6.14±1.59 pg/ml for very mild AD. Meanwhile, the measured concentrations of plasma T-tau were 18.85±10.16 pg/ml for healthy controls, 32.98±10.18 pg/ml for MCI due to AD, and 37.54±12.29 pg/ml for very mild AD. A significant difference in plasma p-tau181 was observed between healthy controls and MCI due to AD (p < 0.001) and between MCI due to AD and very mild AD (p < 0.001). However, for the plasma T-tau concentration, a significant difference existed only between healthy controls and MCI due to AD (p < 0.001). This implies that the plasma p-tau181 level is correlated more to AD severity than plasma T-tau is. Additionally, p-tau181 was observed as approximately 14% of T-tau in human plasma. ? 2018 - IOS Press and the authors. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85048104597&doi=10.3233%2fJAD-170810&partnerID=40&md5=360cc4c2050b7dada08353a0273b167e https://scholars.lib.ntu.edu.tw/handle/123456789/519459 |
ISSN: | 1387-2877 | DOI: | 10.3233/JAD-170810 | SDG/關鍵字: | amyloid beta protein[1-42]; creatinine; hemoglobin A1c; tau protein; threonine; threonine 181; unclassified drug; amyloid beta protein; biological marker; tau protein; Alzheimer disease; Article; blood sampling; clinical practice; comparative study; controlled study; electrocardiography; human; human cell; immunomagnetic separation; limit of detection; medical history; Mini Mental State Examination; neuropsychological test; nuclear magnetic resonance imaging; priority journal; protein determination; protein phosphorylation; protein synthesis; Wechsler adult intelligence scale; Wechsler memory scale; aged; Alzheimer disease; blood; case control study; female; male; middle aged; phosphorylation; very elderly; Aged; Aged, 80 and over; Alzheimer Disease; Amyloid beta-Peptides; Biomarkers; Case-Control Studies; Female; Humans; Magnetic Resonance Imaging; Male; Middle Aged; Neuropsychological Tests; Phosphorylation; tau Proteins |
顯示於: | 醫學系 |
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