https://scholars.lib.ntu.edu.tw/handle/123456789/520415
標題: | A simple and efficient feeder-free culture system to up-scale iPSCs on polymeric material surface for use in 3D bioprinting | 作者: | Wong, C.-W. YOU-TZUNG CHEN CHUNG-LIANG CHIEN Yu, T.-Y. Rwei, S.-P. SHAN-HUI HSU |
公開日期: | 2018 | 出版社: | Elsevier Ltd | 卷: | 82 | 起(迄)頁: | 69-79 | 來源出版物: | Materials Science and Engineering C | 摘要: | The 3D bioprinting and cell/tissue printing techniques open new possibilities for future applications. To facilitate the 3D bioprinting process, a large amount of living cells are required. Induced pluripotent stem cells (iPSCs) represent a promising cell source for bioprinting. However, the maintenance and expansion of undifferentiated iPSCs are expensive and time consuming. Therefore, in this study a culture method to obtain a sufficient amount of healthy and undifferentiated iPSCs in a short-term period was established. The iPSCs could be passaged for twice on tissue culture polystyrene (TCPS) dish with the conditional medium and could adapt to the feeder-free environment. Feeder-free dishes were further prepared from chitosan, chitosan-hyaluronan, silk fibroin, and polyurethane (PU1 and PU2) two-dimensional substrates. The iPSCs cultured on the chitosan substrates showed a higher proliferation rate without losing the stemness feature. Among the different materials, PU2 could be prepared as a thermoresponsive hydrogel, which was a potential ink for 3D bioprinting. The iPSCs cultured on PU2 substrates well survived when further embedded in PU2 hydrogel. Moreover, PU2 hydrogel printed with iPSCs remained structural integrity. The use of PU2 hydrogel to embed iPSCs reduced the injury to iPSCs by shear stress. These results indicate that iPSCs could be expanded on chitosan or PU2 membranes without the feeder layer and then printed in PU2 hydrogel. The combination of these steps could offer a new possibility for future applications of iPSC-based 3D bioprinting in tissue engineering. ? 2017 Elsevier B.V. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85027699652&doi=10.1016%2fj.msec.2017.08.050&partnerID=40&md5=a8079febdf2133ee8881d0caf53e45a6 https://scholars.lib.ntu.edu.tw/handle/123456789/520415 |
ISSN: | 0928-4931 | DOI: | 10.1016/j.msec.2017.08.050 | SDG/關鍵字: | 3D printers; Cells; Chitin; Chitosan; Cytology; Feeding; Polyurethanes; Shear stress; Stem cells; Tissue; Tissue culture; Tissue engineering; Feeder-free; Future applications; Induced pluripotent stem cells; iPSC; Printing techniques; Proliferation rate; Thermo-responsive hydrogels; Tissue-culture polystyrenes; Hydrogels; chitosan; fibroin; polyethylene glycol dimethacrylate hydrogel; polystyrene derivative; bioprinting; chemistry; cytology; human; induced pluripotent stem cell; metabolism; procedures; three dimensional printing; tissue engineering; Bioprinting; Chitosan; Fibroins; Humans; Hydrogel, Polyethylene Glycol Dimethacrylate; Induced Pluripotent Stem Cells; Polystyrenes; Printing, Three-Dimensional; Tissue Engineering |
顯示於: | 基因體暨蛋白體醫學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。