https://scholars.lib.ntu.edu.tw/handle/123456789/523184
標題: | Conditional antisense-knockdown of zebrafish cardiac troponin C as a new animal model for dilated cardiomyopathy | 作者: | YI-LWUN HO YEN-HUNG LIN Tsai W.-Y. FON-JOU HSIEH Tsai H.-J. |
公開日期: | 2009 | 卷: | 73 | 期: | 9 | 起(迄)頁: | 1691-1697 | 來源出版物: | Circulation Journal | 摘要: | Background: Mutations of cardiac troponin C (cTnC) can cause dilated cardiomyopathy in humans. Methods and Results: Plasmids were constructed such that the reverse tetracycline-controlled transactivator (rtTA) was driven by the cardiac myosin light chain 2 promoter. This heart-specific rtTA bound another bidirectional promoter to express the green fluorescence protein reporter gene and the antisense RNA of cTnC in the presence of doxycycline. A transgenic line of zebrafish (CA17) with cTnC dysfunction was also generated. The heart rates of the embryos in the CA17 line were significantly slower than those of embryos in the control T03 transgenic line at 6 and 12 days post fertilization (dpf). In the CA17 line, cardiac chambers in the F2 embryos were significantly greater and the ventricular ejection fraction was lower than those in the T03 at both 6 and 12 dpf. The mortality rate of F2 adult fish of the CA17 line was also significantly higher (P<0.001). Conclusions: Using conditional expression of antisense RNA of zebrafish cTnC, a new animal model with phenotypes simulating dilated cardiomyopathy has been created. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-69549127744&doi=10.1253%2fcircj.CJ-09-0210&partnerID=40&md5=9190a5826610f33e39fa1aa42e246607 https://scholars.lib.ntu.edu.tw/handle/123456789/523184 |
ISSN: | 1346-9843 | DOI: | 10.1253/circj.CJ-09-0210 | SDG/關鍵字: | cardiac myosin; complementary RNA; doxycycline; green fluorescent protein; myosin light chain 2; tetracycline; troponin C; animal experiment; animal model; article; congestive cardiomyopathy; controlled study; embryo; fertilization; gene expression; heart ejection fraction; heart rate; mortality; nonhuman; nucleotide sequence; phenotype; plasmid; promoter region; protein binding; reporter gene; transgenics; zebra fish; Amino Acid Sequence; Animals; Animals, Genetically Modified; Atrial Function; Base Sequence; Blotting, Western; Cardiac Myosins; Cardiomyopathy, Dilated; Disease Models, Animal; Gene Knockdown Techniques; Genotype; Green Fluorescent Proteins; Heart Rate; Molecular Sequence Data; Myocardial Contraction; Myocardium; Myosin Light Chains; Phenotype; Promoter Regions, Genetic; RNA, Antisense; Stroke Volume; Transcription, Genetic; Troponin C; Ventricular Function; Zebrafish; Zebrafish Proteins |
顯示於: | 醫學系 |
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