https://scholars.lib.ntu.edu.tw/handle/123456789/523888
標題: | Connective tissue growth factor and its role in lung adenocarcinoma invasion and metastasis | 作者: | Chang C.-C. JIN-YUAN SHIH YUNG-MING JENG Su J.-L. Lin B.-Z. Chen S.-T. Chau Y.-P. PAN-CHYR YANG Kuo M.-L. |
公開日期: | 2004 | 出版社: | Oxford University Press | 卷: | 96 | 期: | 5 | 起(迄)頁: | 364-375 | 來源出版物: | Journal of the National Cancer Institute | 摘要: | Background: Tumor invasion and metastasis cause most deaths in cancer patients. Connective tissue growth factor (CTGF), a secreted protein that binds to integrins, modulates the invasive behavior of certain human cancer cells, but few mechanistic details are known. We investigated the roles of CTGF and collapsin response mediator protein 1 (CRMP-1) in metastasis and invasion of human lung adenocarcinoma. Methods: We compared vector control-transfected cells with corresponding CTGF gene-transfected cells. Invasive activity was measured with a modified Boyden chamber assay, and metastatic activity was measured in an animal model. We used CTGF deletion mutants, CTGF and CRMP-1 antisense oligonucleotides, and anti-integrin and anti-CRMP-1 antibodies to investigate the functional relationship between CTGF and CRMP-1. Expression of CTGF protein in 78 lung adenocarcinoma specimens was investigated immunohistochemically. All statistical tests were two-sided. Results: Invasive (both P<.001) and metastatic (P<.001 and P = .003, respectively) activities were lower in cells that overexpress CTGF than in vector control cells. Expression of CRMP-1 was higher in CTGF-transfected clones than in vector control cells, and its level decreased after cells were treated with anti-integrin αvβ3 and αvβ5 antibodies. Reduced levels of CRMP-1 protein after the transfection of CRMP-1-specific antisense oligonucleotides, but not sense oligonucleotides, increased the invasiveness of CTGF-transfected cells (mean numbers of invasive CTGF-transfected cells treated with 20 μM CRMP-1-specific sense and antisense oligonucleotides were 327 and 516 cells, respectively [difference = 189 cells, 95% confidence interval {CI} = 156 to 221 cells; P<.001]). The CT module of CTGF was the region primarily responsible for the increased expression of CRMP-1 and the inhibition of invasion (mean numbers of invasive cells expressing full-length CTGF and CT module-deleted mutant were 148 and 385 cells, respectively [difference = 237 cells, 95% CI = 208 to 266 cells; P<.001]). Reduced expression of CTGF in lung cancer specimens was statistically significantly associated with the risk of more advanced-stage disease (stages III and IV versus stages I and II; P = .001), lymph node metastasis (P = .014), and shorter survival (median survival with high levels of CTGF = 66.7 months and median survival for low levels = 18.2 months; difference = 48.5 months, 95% CI = 33.5 to 63.5 months; P = .02. Conclusion: CTGF inhibits metastasis and invasion of human lung adenocarcinoma by a CRMP-1-dependent mechanism. ? Oxford University Press 2004, all rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-1542288151&doi=10.1093%2fjnci%2fdjh059&partnerID=40&md5=f6e59fd5ebde89f549ed563d4b637dcf https://scholars.lib.ntu.edu.tw/handle/123456789/523888 |
ISSN: | 0027-8874 | DOI: | 10.1093/jnci/djh059 | SDG/關鍵字: | antisense oligonucleotide; collapsin response mediator protein 1; connective tissue growth factor; integrin; protein; protein antibody; unclassified drug; collapsin response mediator protein 1; collapsin response mediator protein-1; connective tissue growth factor; immediate early protein; nerve protein; phosphoprotein; signal peptide; tumor marker; adult; aged; article; cancer cell; cancer invasion; cancer mortality; cancer staging; cause of death; cell clone; cell invasion; cell mutant; clinical article; confidence interval; controlled study; female; gene deletion; genetic transfection; human; human cell; human tissue; immunohistochemistry; lung adenocarcinoma; lymph node metastasis; male; metastasis; priority journal; protein binding; protein expression; protein function; statistical analysis; statistical significance; survival time; vector control; adenocarcinoma; cancer invasion; disease course; gene expression regulation; genetics; lung tumor; lymph node metastasis; metabolism; metastasis; mutation; pathology; plasmid; proportional hazards model; reverse transcription polymerase chain reaction; survival; tumor cell line; upregulation; Adenocarcinoma; Cell Line, Tumor; Disease Progression; Gene Deletion; Gene Expression Regulation, Neoplastic; Humans; Immediate-Early Proteins; Immunohistochemistry; Intercellular Signaling Peptides and Proteins; Lung Neoplasms; Lymphatic Metastasis; Mutation; Neoplasm Invasiveness; Nerve Tissue Proteins; Oligonucleotides, Antisense; Phosphoproteins; Plasmids; Proportional Hazards Models; Reverse Transcriptase Polymerase Chain Reaction; Survival Analysis; Transfection; Tumor Markers, Biological; Up-Regulation |
顯示於: | 醫學系 |
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