https://scholars.lib.ntu.edu.tw/handle/123456789/523920
標題: | Up-regulation of tumor interleukin-8 expression by infiltrating macrophages: Its correlation with tumor angiogenesis and patient survival in non-small cell lung cancer | 作者: | Chen J.J.W. Yao P.-L. ANG YUAN Hong T.-M. CHIA-TUNG SHUN Kuo M.-L. Lee Y.-C. PAN-CHYR YANG |
公開日期: | 2003 | 卷: | 9 | 期: | 2 | 起(迄)頁: | 729-737 | 來源出版物: | Clinical Cancer Research | 摘要: | Purpose: To evaluate the interaction between tumorinfiltrating macrophages and cancer cells and its effect on the expression of a potent angiogenic factor, interleukin-8 (IL-8), tumor angiogenesis, and patient outcome in nonsmall cell lung cancer (NSCLC). Experimental Design: We measured tumor IL-8 mRNA expression (by real-time quantitative reverse transcriptionPCR), intratumor microvessel counts, and tumor-infiltrating macrophage density (by immunohistochemical staining) in 35 NSCLC surgical specimens and correlated with the patient's clinical outcome. We then investigated the interaction between macrophages (cell line THP-1) and six different human cancer cell lines (four NSCLCs, one osteosarcoma, and one hepatoma) and its effect on IL-8 mRNA expression using a macrophage/cancer cell coculture system, IL-8 mRNA expression in lung cancer cells, and macrophages being measured separately after coculture in the presence or absence of six anti-inflammatory agents, i.e., pentoxifylline, aspirin, indomethacin, dexamethasone, celecoxib (a selective cyclooxygenase-2 inhibitor), and pyrrolidine dithiocarbamate, a specific nuclear factor κB (NF-κB) inhibitor. NF-κB transcriptional activity and protein levels were measured by reporter gene assay and Western blot. Results: The tumor-infiltrating macrophage density correlated significantly and positively with tumor IL-8 mRNA expression and intratumor microvessel counts and significantly and negatively with patient survival. In addition, after cell-cell interaction in cancer cell:macrophage cocultures, marked IL-8 mRNA expression was induced in lung cancer cells (?270-fold) and, to a lesser degree, in macrophages (4.5-fold). The increase in IL-8 mRNA expression correlated with the in vitro metastatic potential of the cancer cells. All six anti-inflammatory agents suppressed induction of IL-8 mRNA expression in lung cancer cells by > 90%, four (pentoxifylline, celecoxib, pyrrolidine dithiocarbamate, and dexamethasone) having a dose-dependent effect. NF-κB transcriptional regulation and protein levels were simultaneously increased in the nuclei of cancer cells in macrophage/cancer cell cocultures, this effect also being suppressed by all six anti-inflammatory agents. Conclusions: The interaction between infiltrating macrophages and cancer cells up-regulates IL-8 mRNA expression, especially in the cancer cells; this may contribute greatly to the increased tumor angiogenesis and adverse outcome in NSCLC patients with a high density of tumorinfiltrating macrophages. Anti-inflammatory agents can suppress the induction of IL-8 mRNA expression seen in lung cancer cells after coculture with macrophages, and this suppression is mediated, in part, through the NF-κB pathway. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037314662&partnerID=40&md5=0db1ee673b4d092a3316482da1827a7c https://scholars.lib.ntu.edu.tw/handle/123456789/523920 |
ISSN: | 1078-0432 | SDG/關鍵字: | acetylsalicylic acid; celecoxib; dexamethasone; immunoglobulin enhancer binding protein; indometacin; interleukin 8; messenger RNA; pentoxifylline; pyrrolidine dithiocarbamate; adult; angiogenesis; article; cancer cell culture; cancer survival; cell density; cell infiltration; cell interaction; cell level; clinical article; coculture; controlled study; correlation analysis; dose response; female; gene expression regulation; gene induction; hepatoma cell; human; human cell; human tissue; immunohistochemistry; lung non small cell cancer; macrophage; male; metastasis potential; microvasculature; osteosarcoma cell; outcomes research; priority journal; protein expression; protein function; reporter gene; reverse transcription polymerase chain reaction; transcription regulation; tumor vascularization; upregulation; Western blotting; Carcinoma, Non-Small-Cell Lung; Cell Line; Coculture Techniques; Female; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; Interleukin-8; Lung Neoplasms; Macrophages; Male; Microcirculation; Middle Aged; Neovascularization, Pathologic; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Survival Analysis; Transcription, Genetic; Tumor Cells, Cultured |
顯示於: | 醫學系 |
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