https://scholars.lib.ntu.edu.tw/handle/123456789/523932
標題: | Induction of cytochromes P450 1A1 and 1B1 in human lung adenocarcinoma CL5 cells by frying-meat emission particulate | 作者: | Wang H.-W. Chen T.-L. PAN-CHYR YANG Ma Y.-C. Yu C.-C. Ueng T.-H. |
關鍵字: | CYP1A1; CYP1B1; Frying-meat emission particulate; Induction | 公開日期: | 2002 | 卷: | 40 | 期: | 5 | 起(迄)頁: | 653-661 | 來源出版物: | Food and Chemical Toxicology | 摘要: | The effect of airborne frying-meat emission particulate (FMEP) on cytochrome P450 (P450)-dependent monooxygenase was determined using human lung adenocarcinoma cell line CL5 treated with organic extract of FMEP prepared from beef, fish or pork. Treatment with fish FMEP extract caused greater increases of intracellular peroxide production and glutathione content than did beef and pork FMEP extracts. Treatment with 200 μg/ml beef, fish or pork FMEP extract for 6 h increased benzo[a]pyrene hydroxylase, 7-ethoxyresorufin and methoxyresorufin O-dealkylases activities in S9. Immunoblot analysis of S9 proteins from control cells and cells treated with FMEP extracts revealed that the airborne particulates increased proteins immunorelated to CYP1A1 and CYP1B1. Northern blot analysis of total cellular RNA from controls and cells treated with FMEP extracts showed that the cooking by-products increased the levels of CYP1A1 and CYP1B1 mRNA. Treatment with 1 μM dibenzo[a,h]anthracene for 6 h increased monooxygenase activities, CYP1A1 and CYP1B1 protein and mRNA levels in CL5 cells. Beef FMEP extract and dibenzo[a,h]anthracene also induced CYP1A1 and CYP1B1 in human lung carcinoma NCI-H322 cells. The present finding demonstrates that airborne particulates generated during the frying of beef, fish and pork can induce carcinogen-metabolizing CYP1A1 and CYP1B1 in the human lung-derived cell line CL5. ? 2002 Elsevier Science Ltd. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036226191&doi=10.1016%2fS0278-6915%2802%2900008-X&partnerID=40&md5=cb25d71597cffcb737fe10bbd6b58e76 https://scholars.lib.ntu.edu.tw/handle/123456789/523932 |
ISSN: | 0278-6915 | DOI: | 10.1016/S0278-6915(02)00008-X | SDG/關鍵字: | carcinogen; cytochrome P450; cytochrome P450 1A1; cytochrome P450 1B1; dibenz[a,h]anthracene; ethoxyresorufin; glutathione; messenger RNA; peroxide; unspecific monooxygenase; article; beef cattle; cancer cell culture; carcinogenesis; cell line; controlled study; cooking; enzyme activity; female; fish; frying; human; human cell; immunoblotting; lung adenocarcinoma; Northern blotting; Adenocarcinoma; Animals; Aryl Hydrocarbon Hydroxylases; Benz(a)Anthracenes; Blotting, Northern; Carcinogens; Cattle; Cell Line; Cell Survival; Cookery; Cytochrome P-450 CYP1A1; Cytochrome P-450 Enzyme System; Electrophoresis, Polyacrylamide Gel; Environmental Exposure; Fishes; Gas Chromatography-Mass Spectrometry; Humans; Lung Neoplasms; Meat; RNA, Messenger; Subcellular Fractions; Swine; Tumor Cells, Cultured |
顯示於: | 醫學系 |
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