https://scholars.lib.ntu.edu.tw/handle/123456789/524051
Title: | Mucin mRNA expression in lung adenocarcinoma cell lines and tissues | Authors: | CHONG-JEN YU PAN-CHYR YANG Shew J.-Y. Hong T.-M. Yang S.-C. Lee Y.-C. LI-NA LEE KWEN-TAY LUH Wu C.-W. |
Issue Date: | 1996 | Journal Volume: | 53 | Journal Issue: | 2 | Start page/Pages: | 118-126 | Source: | Oncology (Switzerland) | Abstract: | The expression pattern of mucin genes was studied in 7 lung adenocarcinoma cell lines (CL1, CL2, CL3, NCL2, PC9, PC13, PC14) and 12 lung adenocarcinoma tissues. CL1 and PC 13 are poorly differentiated cell lines with low mucin glycoprotein production. The other 5 cell lines are well differentiated and produce a higher amount of mucins. Total RNA was extracted from these cell lines. Northern blot analysis was performed by hybridization with specific antisense oligonucleotide probes recognizing mucin-specific tandem repeats of 4 mucin genes (MUC1, MUC2, MUC3, MUC4). RT-PCR was carried out to amplify the 3′ and 5′ nonrepetitive coding regions of MUC1 and the 5’ nonrepetitive coding region of MUC2. All these cell lines expressed MUC1, MUC2, MUC3, and MUC4 mRNA but in variable mounts. The poorly differentiated cell lines (CL1 and PC 13) had a relatively low level of expression of MUC1, MUC2, MUC3 and MUC4. RT-PCR, with primers amplifying the MUC1 nonrepetitive coding region 5′ end, 293 bp, and the 3′ end, 522 bp, as well as the MUC2 nonrepetitive 5′ coding region, 308 bp, revealed the presence of MUC1 and MUC2 mRNA in all the cell lines. Sequence analysis of the PCR products were very homologous, similar to previously published MUC1 and MUC2 cDNA sequences. The expression pattern of mucin genes is consistent with that of mucin glycoproteins as studied using biochemical and immunological methods. Northern blotting and RT-PCR analysis in 12 lung adenocarcinoma tissues with various grades of differentiation (6 poorly differentiated adenocarcinomas and 6 moderately to well-differentiated adenocarcinomas) showed heterogeneous expression of the 4 mucin genes in tissues without clear correlation with the differentiation grade. Therefore the clinical implications of the differential expression of the mucin genes need further investigation. ? 1996 S. Karger AG, Basel. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0029997542&doi=10.1159%2f000227547&partnerID=40&md5=581e828c107cb92114e64df52f3baaf6 https://scholars.lib.ntu.edu.tw/handle/123456789/524051 |
ISSN: | 0030-2414 | DOI: | 10.1159/000227547 | SDG/Keyword: | complementary dna; glycoprotein; messenger rna; mucin; article; cell differentiation; coding; controlled study; gene expression; human; human cell; human tissue; lung adenocarcinoma; northern blotting; oligonucleotide probe; priority journal; reverse transcription polymerase chain reaction; tandem repeat; Adenocarcinoma; Base Sequence; Blotting, Northern; Gene Expression Regulation, Neoplastic; Humans; Lung Neoplasms; Molecular Sequence Data; Mucins; Neoplasm Proteins; Polymerase Chain Reaction; RNA, Messenger; Tumor Cells, Cultured |
Appears in Collections: | 醫學系 |
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