https://scholars.lib.ntu.edu.tw/handle/123456789/524920
標題: | Efficient generation of chemically induced mesenchymal stem cells from human dermal fibroblasts | 作者: | Lai P.-L. Lin H. Chen S.-F. Yang S.-C. Hung K.-H. Chang C.-F. Chang H.-Y. FRANK LEIGH LU Lee Y.-H. Liu Y.-C. Huang H.-C. HSIAO-CHUN HUANG |
公開日期: | 2017 | 出版社: | Nature Publishing Group | 卷: | 7 | 起(迄)頁: | 44534 | 來源出版物: | Scientific Reports | 摘要: | Human mesenchymal stromal/stem cells (MSCs) are multipotent and currently undergoing hundreds of clinical trials for disease treatments. To date, no studies have generated induced MSCs from skin fibroblasts with chemicals or growth factors. Here, we established the first chemical method to convert primary human dermal fibroblasts into multipotent, induced MSC-like cells (iMSCs). The conversion method uses a defined cocktail of small molecules and growth factors, and it can achieve efficient conversion with an average rate of 38% in 6 days. The iMSCs have much higher clonogenicity than fibroblasts, and they can be maintained and expanded in regular MSC medium for at least 8 passages and further differentiated into osteoblasts, adipocytes, and chondrocytes. Moreover, the iMSCs can suppress LPS-mediated acute lung injury as effectively as bone marrow-derived mesenchymal stem cells. This finding may greatly benefit stem cell biology, cell therapy, and regenerative medicine. ? The Author(s) 2017. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85015281067&doi=10.1038%2fsrep44534&partnerID=40&md5=8bbc93cbda9a6a2c4484401794e0ce41 https://scholars.lib.ntu.edu.tw/handle/123456789/524920 |
ISSN: | 2045-2322 | DOI: | 10.1038/srep44534 | SDG/關鍵字: | signal peptide; cell differentiation; chondrocyte; cytology; drug effect; fibroblast; human; induced pluripotent stem cell; mesenchymal stem cell transplantation; mesenchymal stroma cell; molecular library; pharmacology; skin; Cell Differentiation; Chondrocytes; Fibroblasts; Humans; Induced Pluripotent Stem Cells; Intercellular Signaling Peptides and Proteins; Mesenchymal Stem Cell Transplantation; Mesenchymal Stromal Cells; Skin; Small Molecule Libraries |
顯示於: | 醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。