https://scholars.lib.ntu.edu.tw/handle/123456789/525203
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Yu, Chih-Hsiang | en_US |
dc.contributor.author | Lin, Tze-Kang | en_US |
dc.contributor.author | SHIANN-TANG JOU | en_US |
dc.contributor.author | Lin, Chien-Yu | en_US |
dc.contributor.author | Lin, Kai-Hsin | en_US |
dc.contributor.author | MENG-YAO LU | en_US |
dc.contributor.author | Chen, Shu-Huey | en_US |
dc.contributor.author | Cheng, Chao-Neng | en_US |
dc.contributor.author | Wu, Kang-Hsi | en_US |
dc.contributor.author | Wang, Shih-Chung | en_US |
dc.contributor.author | HSIU-HAO CHANG | en_US |
dc.contributor.author | MENG-JU LI | en_US |
dc.contributor.author | Ni, Yu-Ling | en_US |
dc.contributor.author | Su, Yi-Ning | en_US |
dc.contributor.author | Lin, Dong-Tsamn | en_US |
dc.contributor.author | Chen, Hsuan-Yu | en_US |
dc.contributor.author | Harrison, Christine J | en_US |
dc.contributor.author | Hung, Chia-Cheng | en_US |
dc.contributor.author | SHU-WHA LIN | en_US |
dc.contributor.author | YUNG-LI YANG | en_US |
dc.date.accessioned | 2020-12-09T02:07:49Z | - |
dc.date.available | 2020-12-09T02:07:49Z | - |
dc.date.issued | 2020 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85087921249&doi=10.1038%2fs41598-020-68311-9&partnerID=40&md5=994f120f4a69fc85e2aafcc82a69a954 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/525203 | - |
dc.description.abstract | Aneuploidy occurs within a significant proportion of childhood B-cell acute lymphoblastic leukemia (B-ALL). Some copy number variations (CNV), associated with novel subtypes of childhood B-ALL, have prognostic significance. A total of 233 childhood B-ALL patients were enrolled into this study. Focal copy number alterations of ERG, IKZF1, PAX5, ETV6, RB1, BTG1, EBF1, CDKN2A/2B, and the Xp22.33/Yp11.31 region were assessed by Multiplex Ligation-dependent Probe Amplification (MLPA). The MLPA telomere kit was used to identify aneuploidy through detection of whole chromosome loss or gain. We carried out these procedures alongside measurement of DNA index in order to identify, aneuploidy status in our cohort. MLPA telomere data and DNA index correlated well with aneuploidy status at higher sensitivity than cytogenetic analysis. Three masked hypodiploid patients, undetected by cytogenetics, and their associated copy number neutral loss of heterozygosity (CN-LOH) were identified by STR and SNP arrays. Rearrangements of TCF3, located to 19p, were frequently associated with 19p deletions. Other genetic alterations including iAMP21, IKZF1 deletions, ERG deletions, PAX5AMP, which have clinical significance or are associated with novel subtypes of ALL, were identified. In conclusion, appropriate application of MLPA aids the identifications of CNV and aneuploidy in childhood B-ALL. ? 2020, The Author(s). | - |
dc.publisher | Nature Research | - |
dc.relation.ispartof | Scientific Reports | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | DNA; acute lymphoblastic leukemia; adolescent; aneuploidy; B lymphocyte; blood; child; chromosome aberration; copy number variation; female; genetics; human; infant; male; molecular pathology; multiplex polymerase chain reaction; pathology; preschool child; procedures; prognosis; Adolescent; Aneuploidy; B-Lymphocytes; Child; Child, Preschool; Chromosome Aberrations; DNA Copy Number Variations; DNA, Neoplasm; Female; Humans; Infant; Male; Multiplex Polymerase Chain Reaction; Pathology, Molecular; Precursor Cell Lymphoblastic Leukemia-Lymphoma; Prognosis | - |
dc.title | MLPA and DNA index improve the molecular diagnosis of childhood B-cell acute lymphoblastic leukemia | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1038/s41598-020-68311-9 | - |
dc.identifier.pmid | 32661308 | - |
dc.identifier.scopus | 2-s2.0-85087921249 | - |
dc.relation.pages | 11501 | - |
dc.relation.journalvolume | 10 | - |
dc.relation.journalissue | 1 | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Pediatrics-NTUH | - |
crisitem.author.dept | Pediatrics | - |
crisitem.author.dept | Pediatrics-NTUH | - |
crisitem.author.dept | Pediatrics | - |
crisitem.author.dept | Pediatrics | - |
crisitem.author.dept | Pediatrics-NTUH | - |
crisitem.author.dept | Pediatrics-NTUHHC | - |
crisitem.author.dept | Pediatrics-NTUH | - |
crisitem.author.dept | Clinical Laboratory Sciences and Medical Biotechnology | - |
crisitem.author.dept | Pediatrics | - |
crisitem.author.dept | Laboratory Medicine | - |
crisitem.author.dept | Pediatrics-NTUH | - |
crisitem.author.dept | Laboratory Medicine-NTUH | - |
crisitem.author.orcid | 0000-0003-1483-0403 | - |
crisitem.author.orcid | 0000-0003-4461-9967 | - |
crisitem.author.orcid | 0000-0003-1832-8509 | - |
crisitem.author.orcid | 0000-0002-1838-6576 | - |
crisitem.author.orcid | 0000-0001-6748-5581 | - |
crisitem.author.orcid | 0000-0002-3598-7218 | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | NTU Hsin-Chu Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
顯示於: | 醫學系 |
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