https://scholars.lib.ntu.edu.tw/handle/123456789/526014
標題: | Adenoviral interneuronal transportation after retrograde gene transfer in mice | 作者: | LI-KAI TSAI Tsai M.-S. Shyue S.-K. WUH-LIANG HWU Li H. |
公開日期: | 2005 | 卷: | 142 | 期: | 2 | 起(迄)頁: | 151-155 | 來源出版物: | Molecular Brain Research | 摘要: | Although retrograde gene transfer from infected muscles to neurons by viral vectors has been known for years, it is still unknown whether interneuronal gene transportation of viral vectors occurs after retrograde gene transfer. To determine this, we injected adenoviral vectors carrying eGFP gene with or without a neural tracer into the right gastrocnemius muscles of mice. After 7 days, some spinal motor neurons were detected with green fluorescence but without the signal of neural tracer. In addition, nerves with green fluorescence could be noted in the right lumbosacral paraspinal muscles of viral-injected mice. The green fluorescence in the right lumbosacral paraspinal muscles might have resulted from retrograde gene transportation from the viral-injected gastrocnemius muscles to the spinal neurons, followed by interneuronal transfer and anterograde expression of eGFP in the axons belonging to neurons innervating the paraspinal muscles. This phenomenon of interneuronal transportation raises the possibility that we could treat motoneuron diseases by injection of viral vectors containing therapeutic genes into a few muscles resulting in widespread beneficial effects. ? 2005 Elsevier B.V. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-27944488006&doi=10.1016%2fj.molbrainres.2005.09.021&partnerID=40&md5=5cdab7463a9c0100ff26f78712371ad1 https://scholars.lib.ntu.edu.tw/handle/123456789/526014 |
ISSN: | 0169-328X | DOI: | 10.1016/j.molbrainres.2005.09.021 | SDG/關鍵字: | adenovirus vector; animal experiment; article; back muscle; gastrocnemius muscle; gene transfer; interneuron; mouse; nonhuman; priority journal; retrograde nerve fiber transport; spinal cord motoneuron; spinal cord nerve cell; Adenoviridae; Animals; Animals, Newborn; Cell Line, Tumor; Choline O-Acetyltransferase; Gene Transfer Techniques; Glial Fibrillary Acidic Protein; Green Fluorescent Proteins; Humans; Mice; Motor Neurons; Muscles; Neuroblastoma; Phosphopyruvate Hydratase; Transgenes; Adenoviridae; Animalia |
顯示於: | 醫學系 |
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