https://scholars.lib.ntu.edu.tw/handle/123456789/527278
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | CHEN-TI WANG | en_US |
dc.contributor.author | YU-TSAN LIN | en_US |
dc.contributor.author | BOR-LUEN CHIANG | en_US |
dc.contributor.author | Lin Y.-H. | en_US |
dc.contributor.author | Hou S.-M. | en_US |
dc.creator | Wang C.-T.;Yu-Tsan Lin;Chiang B.-L.;Lin Y.-H.;Hou S.-M. | - |
dc.date.accessioned | 2020-12-18T01:52:55Z | - |
dc.date.available | 2020-12-18T01:52:55Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 1063-4584 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-33751190978&doi=10.1016%2fj.joca.2006.05.009&partnerID=40&md5=03b9304aba647cf26db82d23bbd6107f | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/527278 | - |
dc.description.abstract | Objective: Activated synoviocytes play important roles in the progression of human osteoarthritis (OA). Intra-articular injection of high molecular weight hyaluronic acid (HMW-HA) has been used as viscosupplementation for knee OA but its effect on synoviocytes remains undisclosed. This study aims to investigate the effects of HMW-HA on the gene expression of 16 OA-associated cytokines and enzymes, including interleukin (IL)-1β, IL-6, IL-8, leukemia inhibitory factor (LIF), tumor necrosis factor (TNF)-α, TNF-α converting enzyme (TACE), matrix metalloproteinase (MMP)-1, MMP-2, MMP-3, MMP-9, MMP-13, tissue inhibitor of metalloproteinase (TIMP)-1, TIMP-2, aggrecanase-1, aggrecanase-2, and inducible nitric oxide synthase (iNOS), in fibroblast-like synoviocytes (FLS) from patients with early stage OA. Method: Synovial fluid-derived FLS were obtained from the knees of 15 patients with early stage OA. IL-1-stimulated or unstimulated FLS were cultured with or without the treatment of 600-800 kDa HMW-HA. Moreover, blocking experiments with anti-CD44 monoclonal antibodies (mAb) were used to examine the involvement of CD44 in HMW-HA effects. We designed and validated the real-time quantitative polymerase chain reaction (Q-PCR) assays with SYBR Green dyes for simultaneous quantification of the expression of the 16 genes. Results: HMW-HA down-regulated IL-8 and iNOS gene expression in unstimulated FLS and down-regulated aggrecanase-2 and TNF-α gene expression in IL-1-stimulated FLS. CD44 blocking inhibited the down-regulatory effects of HMW-HA on gene expression. Conclusion: HMW-HA may have a structure-modifying effect for OA by down-regulation of aggrecanase-2 in FLS. HMW-HA also has an anti-inflammatory effect by down-regulation of TNF-α, IL-8, and iNOS in FLS. These effects may be mediated through the interaction of CD44 and HMW-HA. ? 2006 OsteoArthritis Research Society International. | - |
dc.relation.ispartof | Osteoarthritis and Cartilage | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | aggrecanase; aggrecanase 1; aggrecanase 2; artzdispo; collagenase 3; cytokine; enzyme; gelatinase A; gelatinase B; Hermes antigen; hyaluronic acid; inducible nitric oxide synthase; interleukin 1beta; interleukin 6; interleukin 8; interstitial collagenase; leukemia inhibitory factor; monoclonal antibody; stromelysin; tissue inhibitor of metalloproteinase 1; tissue inhibitor of metalloproteinase 2; tumor necrosis factor alpha; tumor necrosis factor alpha converting enzyme; unclassified drug; antiinflammatory activity; article; cell activation; clinical article; concentration response; controlled study; disease course; down regulation; drug effect; gene amplification; gene expression regulation; human; human cell; knee osteoarthritis; molecular weight; nucleotide sequence; phenotypic variation; priority journal; quantitative analysis; real time polymerase chain reaction; synovial fluid; synoviocyte; validation study; ADAM Proteins; Antigens, CD44; Cytokines; Dose-Response Relationship, Drug; Down-Regulation; Fibroblasts; Humans; Hyaluronic Acid; Interleukin-1; Interleukin-8; Molecular Weight; Nitric Oxide Synthase Type II; Osteoarthritis, Knee; Polymerase Chain Reaction; Synovial Fluid; Tumor Necrosis Factor-alpha | - |
dc.title | High molecular weight hyaluronic acid down-regulates the gene expression of osteoarthritis-associated cytokines and enzymes in fibroblast-like synoviocytes from patients with early osteoarthritis | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1016/j.joca.2006.05.009 | - |
dc.identifier.pmid | 16806998 | - |
dc.identifier.scopus | 2-s2.0-33751190978 | - |
dc.relation.pages | 1237-1247 | - |
dc.relation.journalvolume | 14 | - |
dc.relation.journalissue | 12 | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Orthopedic Surgery-NTUH | - |
crisitem.author.dept | Orthopedic Surgery | - |
crisitem.author.dept | Pediatrics | - |
crisitem.author.dept | Pediatrics-NTUH | - |
crisitem.author.dept | Pediatrics | - |
crisitem.author.dept | Pediatrics-NTUH | - |
crisitem.author.dept | Clinical Medicine | - |
crisitem.author.dept | Oral Biology | - |
crisitem.author.dept | College of Life Science | - |
crisitem.author.dept | Immunology | - |
crisitem.author.orcid | 0000-0002-0246-6846 | - |
crisitem.author.orcid | 0000-0002-4192-3165 | - |
crisitem.author.orcid | 0000-0002-6705-0286 | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University | - |
crisitem.author.parentorg | College of Medicine | - |
顯示於: | 醫學系 |
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