https://scholars.lib.ntu.edu.tw/handle/123456789/527814
Title: | A nanodroplet cell processing platform facilitating drug synergy evaluations for anti-cancer treatments | Authors: | Kuo, C.-T. Wang, J.-Y. Lu, S.-R. Lai, Y.-S. HSIU-HAO CHANG Hsieh, J.-T. Wo, A.M. Chen, B.P.C. Lu, J.-H. ANDREW WO HSIN-YU LEE |
Issue Date: | 2019 | Publisher: | Nature Publishing Group | Journal Volume: | 9 | Journal Issue: | 1 | Start page/Pages: | 10120 | Source: | Scientific Reports | Abstract: | Therapeutic drug synergism intervened in cancer treatments has been demonstrated to be more effective than using a single effector. However, it remains inherently challenging, with a limited cell count from tumor samples, to achieve potent personalized drug cocktails. To address the issue above, we herein present a nanodroplet cell processing platform. The platform incorporates an automatic nanodroplet dispenser with cell array ParaStamp chips, which were fabricated by a new wax stamping approach derived from laser direct writing. Such approach enables not only the on-demand de-wetting with hydrophobic wax films on substrates but also the mask-less fabrication of non-planar microstructures (i.e. no photolithography process). The ParaStamp chip was pre-occupied with anti-cancer drugs and their associate mixtures, enabling for the spatially addressable screening of optimal drug combinations simultaneously. Each droplet with a critical volume of 200 nl containing with 100 cells was utilized. Results revealed that the optimal combination reduces approximate 28-folds of conducted doses compared with single drugs. Tumor inhibition with the optimally selected drug combination was further confirmed by using PC-3 tumor-bearing mouse models. Together, the nanodroplet cell processing platform could therefore offer new opportunities to power the personalized cancer medicine at early-stage drug screening and discovery. ? 2019, The Author(s). |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85069005490&doi=10.1038%2fs41598-019-46502-3&partnerID=40&md5=2d4003669938463d02044ba96b3b40ea https://scholars.lib.ntu.edu.tw/handle/123456789/527814 |
ISSN: | 2045-2322 | DOI: | 10.1038/s41598-019-46502-3 | SDG/Keyword: | antineoplastic agent; baysilon; dimeticone; animal; devices; drug potentiation; drug screening; equipment design; high throughput screening; human; laser; male; miniaturization; nude mouse; procedures; Animals; Antineoplastic Combined Chemotherapy Protocols; Dimethylpolysiloxanes; Drug Screening Assays, Antitumor; Drug Synergism; Equipment Design; High-Throughput Screening Assays; Humans; Lasers; Male; Mice, Nude; Miniaturization; PC-3 Cells; Xenograft Model Antitumor Assays |
Appears in Collections: | 醫學系 |
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