https://scholars.lib.ntu.edu.tw/handle/123456789/529887
標題: | Uropathogenic Escherichia coli alters muscle contractions in rat urinary bladder via a nitric oxide synthase-related signaling pathway | 作者: | TE-I WENG WEN-JONE CHEN Hsiao Y.W. Liu S.-H. |
公開日期: | 2006 | 卷: | 194 | 期: | 12 | 起(迄)頁: | 1774-1782 | 來源出版物: | Journal of Infectious Diseases | 摘要: | Background. Uropathogenic Escherichia coli (UPEC) is a common cause of urinary-tract infection. The mechanisms by which bacteria cause the symptoms of cystitis remain unclear. Methods. The contractions of isolated rat detrusor strips evoked by electrical field stimulations (EFS) or by exogenous agonists and immunoblotting for the detection of protein expression in the bladder were measured in the short (1 h) and long (24 h) term after the intravesical instillation of [96 (O4:K6) strain or UPEC isolated from patients with acute E. coli pyelonephritis or E. coli lipopolysaccharide (LPS). Results. One hour after the instillation of UPEC, the level of endothelial nitric oxide synthase (eNOS) and the contractile response, but not protein kinase C (PKC) and extracellular signal-regulated kinase (ERK)-1/2 activation, were higher. Twenty-four hours after UPEC treatment, detrusor contractions were decreased, and inducible (i) NOS protein expression and ERK1/2 phosphorylation, but not PKC activation, were observed. Both aminoguanidine and PD98059 treatment markedly reversed the decrease of EFS- and acetylcholine-evoked detrusor contractions induced by UPEC. The instillation of LPS triggered PKC activation but not ERK1/2 phosphorylation. Conclusions. Short-term intravesical instillation of UPEC enhances detrusor contractions through an eNOS-related pathway, but iNOS-regulated ERK1/2 signaling may be involved in long-term UPEC treatment-induced responses. There are different mechanisms involved in the responses induced by UPEC and EPS. ? 2006 by the Infectious Diseases Society of America. All rights reserved. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/529887 | ISSN: | 0022-1899 | DOI: | 10.1086/509620 | SDG/關鍵字: | 2 (2 amino 3 methoxyphenyl)chromone; acetylcholine; aminoguanidine; endothelial nitric oxide synthase; inducible nitric oxide synthase; lipopolysaccharide; mitogen activated protein kinase 1; mitogen activated protein kinase 3; n(g) nitroarginine methyl ester; nitric oxide synthase; protein kinase C; animal experiment; animal model; article; bacterial infection; bladder contraction; bladder muscle; controlled study; cystitis; detrusor muscle; enzyme phosphorylation; Escherichia coli; female; Gram negative infection; inflammation; nonhuman; nucleotide sequence; priority journal; protein expression; pyelonephritis; rat; reverse transcription polymerase chain reaction; signal transduction; urinary tract infection; urine level; Western blotting; Animals; Disease Models, Animal; Escherichia coli; Escherichia coli Infections; Female; Mitogen-Activated Protein Kinase 1; Mitogen-Activated Protein Kinase 3; Muscle Contraction; Muscle, Smooth; Nitric Oxide Synthase; Phosphorylation; Rats; Rats, Wistar; Signal Transduction; Urinary Bladder; Urinary Tract Infections |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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