https://scholars.lib.ntu.edu.tw/handle/123456789/532411
標題: | Pentoxifylline inhibits human peritoneal mesothelial cell growth and collagen synthesis: Effects on TGF-β | 作者: | CHENG-CHUNG FANG CHUNG-JEN YEN YUNG-MING CHEN Shyu R.-S. TUN-JUN TSAI PO-HUANG LEE Hsieh B.-S. |
公開日期: | 2000 | 出版社: | Blackwell Publishing Inc. | 卷: | 57 | 期: | 6 | 起(迄)頁: | 2626-2633 | 來源出版物: | Kidney International | 摘要: | Background. Prevention or treatment of peritoneal fibrosing syndrome has become an important issue in patients on continuous ambulatory peritoneal dialysis (CAPD). Recent evidence has suggested that mesothelial stem cell proliferation and matrix over-production predispose the development of peritoneal fibrosis. We investigated whether pentoxifylline (PTX) affects human peritoneal mesothelial cell (HPMC) growth and collagen synthesis. Methods. HPMC was cultured from human omentum by an enzymic disaggregation method. Cell proliferation was assayed using a methyltetrazolium uptake method. Cell cycle analysis was performed by flow cytometry. Collagen synthesis was measured by 5H-proline incorporation into pepsin-resistant, salt-precipitated collagen. Prostaglandins and cAMP were determined by enzyme immunoassay. Northern blot analysis was used to determine mRNA expression. Results. Our data show that PTX inhibited serum-stimulated HPMC growth and collagen synthesis in a dose-dependent manner. Cell cycle analysis showed that PTX arrested the HPMCs in the G1 phase. PTX decreased the procollagen 1/4 (I) mRNA expression either stimulated by serum or transforming growth factor- β (TGF-β). PTX did not alter prostaglandins synthesis but dose-dependently increased intracellular cAMP level. PTX, the same as 3-isobutyl-l- methylxanthine, could potentlate prostaglandin E1 (PGE1) increased cAMP levels of HPMC. The antimitogenic and antifibrogenic effects of PTX on HPMC were reversed by N-[2]-((p-Bromocinnamyl)amino)ethyl]-5- isoquinolinesulfonamide (H-89). Therefore, the mechanism of these effects may be due to the phospodiesterase inhibitory property of PTX. Conclusions. These data suggest that PTX may have a role in treating peritoneal fibrosing syndrome. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0034111904&doi=10.1046%2fj.1523-1755.2000.00123.x&partnerID=40&md5=545b1fd9a44fe8d37a0dc1f60d249e11 https://scholars.lib.ntu.edu.tw/handle/123456789/532411 |
ISSN: | 0085-2538 | DOI: | 10.1046/j.1523-1755.2000.00123.x | SDG/關鍵字: | collagen; cyclic AMP; pentoxifylline; transforming growth factor beta; article; cell culture; cell proliferation; collagen synthesis; continuous ambulatory peritoneal dialysis; controlled study; fibrosis; human; human cell; inflammation; kidney failure; mesothelium cell; Northern blotting; peritoneum cell; priority journal |
顯示於: | 醫學系 |
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