https://scholars.lib.ntu.edu.tw/handle/123456789/532726
標題: | Patients with idiopathic pulmonary nontuberculous mycobacterial disease have normal Th1/Th2 cytokine responses but diminished Th17 cytokine and enhanced granulocyte-macrophage colony-stimulating factor production | 作者: | UN-IN WU Olivier K.N. Kuhns D.B. Fink D.L. Sampaio E.P. Zelazny A.M. Shallom S.J. Marciano B.E. Lionakis M.S. Holland S.M. |
關鍵字: | Cystic fibrosis; Cytokines; Nontuberculous mycobacteria; PNTM; Primary ciliary dyskinesia; Th17 | 公開日期: | 2019 | 出版社: | Oxford University Press | 卷: | 6 | 期: | 12 | 來源出版物: | Open Forum Infectious Diseases | 摘要: | Objective. Although disseminated nontuberculous mycobacterial infection is attributed to defects in the interleukin (IL)-12/interferon-γ circuit, the immunophenotype of idiopathic pulmonary nontuberculous mycobacterial (PNTM) disease is not well defined. Method. We phenotyped Th1, Th2, Th17, and Treg cytokines and colony-stimulating factor production from patients with idiopathic PNTM disease. Data were compared with healthy donors, cystic fibrosis (CF), and primary ciliary dyskinesia (PCD) patients with PNTM disease. Both supernatant cytokine production and intracellular cytokines expressed by various leukocyte subpopulations following mitogen and antigen stimulation were assayed by electrochemiluminescence–based multiplex immunoassay and flow cytometry, respectively. Results. Regardless of antigen or mitogen stimulation, neither intracellular nor extracellular Th1, Th2, and Treg cytokine levels differed between patients and controls. Th17 cells and IL-17A levels were lower in idiopathic PNTM patients, whereas monocyte granulocyte-macrophage colony-stimulating factor (GM-CSF) expression in response to NTM stimulation was higher compared with healthy donors. Besides, distinct cytokine responses following stimulation by Mycobacterium abscessus and Mycobacterium avium were observed consistently within each group. Conclusions. The IL-12/IFN-γ circuit appeared intact in patients with idiopathic PNTM disease. However, idiopathic PNTM patients had reduced Th17 response and higher mycobacteria-induced monocyte GM-CSF expression. ? 2019 Oxford University Press. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85079124916&doi=10.1093%2fofid%2fofz484&partnerID=40&md5=29dd11f16f95b60d46701a2adb76f63c https://scholars.lib.ntu.edu.tw/handle/123456789/532726 |
ISSN: | 2328-8957 | DOI: | 10.1093/ofid/ofz484 | SDG/關鍵字: | cytokine; gamma interferon; granulocyte macrophage colony stimulating factor; interleukin 10; interleukin 12; interleukin 13; interleukin 17; interleukin 2; interleukin 4; tumor necrosis factor; adult; aged; Article; atypical mycobacteriosis; blood sampling; cell culture technique; ciliary dyskinesia; clinical article; controlled study; cystic fibrosis; cytokine production; electrochemiluminescence; female; flow cytometry; human; human cell; idiopathic pulmonary nontuberculous mycobacterial disease; immunoassay; immunophenotyping; lung disease; male; Mycobacterium abscessus; Mycobacterium avium; nonhuman; peripheral blood mononuclear cell; phenotype; priority journal; protein expression; regulatory T lymphocyte; Th1 Th2 balance; Th17 cell |
顯示於: | 醫學系 |
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