https://scholars.lib.ntu.edu.tw/handle/123456789/535219
標題: | High and increasing Oxa-51 DNA load predict mortality in acinetobacter baumannii bacteremia: Implication for pathogenesis and evaluation of therapy | 作者: | YU-CHUNG CHUANG SHAN-CHWEN CHANG Wang W.-K. |
公開日期: | 2010 | 卷: | 5 | 期: | 11 | 起(迄)頁: | e14133 | 來源出版物: | PLoS ONE | 摘要: | Background: While quantification of viral loads has been successfully employed in clinical medicine and has provided valuable insights and useful markers for several viral diseases, the potential of measuring bacterial DNA load to predict outcome or monitor therapeutic responses remains largely unexplored. We tested this possibility by investigating bacterial loads in Acinetobacter baumannii bacteremia, a rapidly increasing nosocomial infection characterized by high mortality, drug resistance, multiple and complicated risk factors, all of which urged the need of good markers to evaluate therapeutics. Methods and Findings: We established a quantitative real-time PCR assay based on an A. baumannii-specific gene, Oxa-51, and conducted a prospective study to examine A. baumannii loads in 318 sequential blood samples from 51 adults patients (17 survivors, 34 nonsurvivors) with culture-proven A. baumannii bacteremia in the intensive care units. Oxa-51 DNA loads were significantly higher in the nonsurvivors than survivors on day 1, 2 and 3 (P = 0.03, 0.001 and 0.006, respectively). Compared with survivors, nonsurvivors had higher maximum Oxa-51 DNA load and a trend of increase from day 0 to day 3 (P<0.001), which together with Pitt bacteremia score were independent predictors for mortality by multivariate analysis (P = 0.014 and 0.016, for maximum Oxa-51 DNA and change of Oxa-51 DNA, respectively). Kaplan-Meier analysis revealed significantly different survival curves in patients with different maximum Oxa-51 DNA and change of Oxa-51 DNA from day 0 to day 3. Conclusions: High Oxa-51 DNA load and its initial increase could predict mortality. Moreover, monitoring Oxa-51 DNA load in blood may provide direct parameters for evaluating new regimens against A. baumannii in future clinical studies. ? 2010 Chuang et al. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-78649798530&doi=10.1371%2fjournal.pone.0014133&partnerID=40&md5=0a199367966b77ba7428e1044eaceabb https://scholars.lib.ntu.edu.tw/handle/123456789/535219 |
ISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0014133 | SDG/關鍵字: | bacterial DNA; bacterial protein; Oxa 51 protein; unclassified drug; antiinfective agent; bacterial DNA; bacterial protein; Acinetobacter baumannii; Acinetobacter infection; adult; aged; antibiotic therapy; article; bacteremia; blood sampling; controlled study; female; human; male; mortality; nonhuman; pathogenesis; prediction; prospective study; quantitative analysis; real time polymerase chain reaction; Acinetobacter baumannii; Acinetobacter infection; bacteremia; bacterial gene; blood; drug effect; genetics; Kaplan Meier method; microbiology; middle aged; polymerase chain reaction; prognosis; proportional hazards model; statistics; survival rate; Acinetobacter baumannii; Bacteria (microorganisms); Acinetobacter baumannii; Acinetobacter Infections; Aged; Anti-Bacterial Agents; Bacteremia; Bacterial Proteins; DNA, Bacterial; Female; Genes, Bacterial; Humans; Kaplan-Meier Estimate; Male; Middle Aged; Polymerase Chain Reaction; Prognosis; Proportional Hazards Models; Survival Rate |
顯示於: | 醫學系 |
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