https://scholars.lib.ntu.edu.tw/handle/123456789/539650
Title: | Cytokine release of peripheral blood mononuclear cells in children with chronic hepatitis B virus infection | Authors: | HONG-YUAN HSU MEI-HWEI CHANG YEN-HSUAN NI PING-ING LEE |
Issue Date: | 1999 | Journal Volume: | 29 | Journal Issue: | 5 | Start page/Pages: | 540-545 | Source: | Journal of Pediatric Gastroenterology and Nutrition | Abstract: | Background: Immune response to hepatitis B virus (HBV) antigens or mitogens in Asian children with chronic HBV infection who are mainly perinatally infected has not been studied in connection with the production of various cytokines, although these patients are considered to be less responsive to antiviral therapy. Methods: The production of the cytokines interferon (IFN)-γ, lymphotoxin, interleukin (IL)-4, tumor necrosis factor (TNF)-α, and interleukin (IL)-1β by peripheral blood mononuclear cells (PBMCs) was studied in 17 hepatitis B surface antigen (HBsAg) carrier children with raised alanine transferase levels (group 1), 17 HBsAg carrier children with normal alanine transferase levels (group 2), and 20 healthy noncarrier control subjects (group 3). Results: Hepatitis B core antigen (HBcAg)-stimulated IFN-α production was significantly higher in group 1 than in groups 2 and 3, serum HBcAg cleared within 1 year in five of eight children in group 1 with stimulation indexes higher than 3, and HBcAg-induced IL-4 secretion was minimal in all groups. Interferon-γ produced by PBMCs stimulated by purified HBsAg did not differ among the three groups. Higher lymphotoxin production by PBMCs stimulated by HBcAg was also noted in groups 1 and 2 than in group 3. Lipopolysaccharide (LPS)-stimulated TNF-α production by PBMCs was significantly higher in group 1 than in group 2. There was no association between HBcAg-anti-HBe status and production of various cytokines. No differences were seen in the profile of cytokines induced by HBV antigens or LPS in children of carrier mothers compared with children of HBsAg-negative mothers. Conclusion: Increased IFN-γ production resulting from HBcAg-specific T-helper lymphocyte type 1 response, and increased TNF-α production may contribute to cell-mediated antiviral immune response in children with chronic hepatitis B. In HBV carrier children, the ability to produce the studied cytokines is related to whether an endogenous immune attempt to eliminate HBV infection emerges in the patients but is not related to the different modes of acquisition of HBV infection. (C) 1999 Lippincott Williams and Wilkins, Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0033512285&doi=10.1097%2f00005176-199911000-00013&partnerID=40&md5=e1953fd4acb9a46989eb9ef199a203a8 https://scholars.lib.ntu.edu.tw/handle/123456789/539650 |
ISSN: | 0277-2116 | DOI: | 10.1097/00005176-199911000-00013 | SDG/Keyword: | alanine aminotransferase; gamma interferon; hepatitis B core antigen; hepatitis B surface antigen; hepatitis B(e) antigen; interleukin 1beta; interleukin 4; lipopolysaccharide; lymphotoxin; tumor necrosis factor alpha; adolescent; article; cell interaction; child; chronic hepatitis; clinical article; clinical trial; controlled clinical trial; controlled study; cytokine production; cytokine release; female; helper cell; Hepatitis B virus; human; human cell; human tissue; immune response; male; priority journal; randomized controlled trial; Adolescent; Child; Child, Preschool; Cytokines; Female; Hepatitis B Core Antigens; Hepatitis B Surface Antigens; Hepatitis B, Chronic; Humans; Interferon Type II; Interleukin-1; Interleukin-4; Leukocytes, Mononuclear; Lipopolysaccharides; Lymphotoxin-alpha; Male; Tumor Necrosis Factor-alpha; Hepatitis B virus |
Appears in Collections: | 醫學系 |
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