https://scholars.lib.ntu.edu.tw/handle/123456789/539908
Title: | Use of recombinant flagellin in oil-in-water emulsions enhances hemagglutinin-specific mucosal IgA production and IL-17 secreting T cells against H5N1 avian influenza virus infection | Authors: | Lai C.-H. Tang N. Jan J.-T. Huang M.-H. CHUN-YI LU BOR-LUEN CHIANG LI-MIN HUANG Wu S.-C. |
Issue Date: | 2015 | Publisher: | Elsevier Ltd | Journal Volume: | 33 | Journal Issue: | 35 | Start page/Pages: | 4321-4329 | Source: | Vaccine | Abstract: | Researchers are currently involved in a strong effort to find a safe and effective vaccine against highly pathogenic avian influenza H5N1 viruses. Toward that goal, we obtained soluble recombinant flagellin (FliC) of Salmonella typhimurium to be used as a mucosal adjuvant for H5HA subunit vaccine development. Intranasal immunization of H5HA antigen with recombinant FliC protein in an oil-in-water emulsion increased H5HA-specific IgG and IgA titers in sera, bronchoalveolar lavage fluids (BALFs), and nasal washes. Use of FliC adjuvant for intranasal immunization further augmented B-cell responses in mucosal environments via increased IgA titers in BALFs and nasal washes. Increases in IgA and IgG titers through the use of FliC adjuvant in intranasal immunization correlated with higher neutralizing antibody titers in sera and BALFs and higher numbers of IgG- and IgA-secreting B cells in spleen and cervical lymph nodes. High levels of IL-17A cytokine production were also found in stimulated T cells of spleen and cervical lymph node cells, only by intranasal immunization particularly with the use of FliC adjuvant in oil-in-water emulsions. These findings may provide useful information toward the development of H5HA mucosal influenza vaccines. ? 2015 Elsevier Ltd. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84939574682&doi=10.1016%2fj.vaccine.2015.03.082&partnerID=40&md5=0c8d4551330d50301d21dfa929341c37 https://scholars.lib.ntu.edu.tw/handle/123456789/539908 |
ISSN: | 0264-410X | DOI: | 10.1016/j.vaccine.2015.03.082 | SDG/Keyword: | avian influenza vaccine; flagellin; hemagglutinin; immunoglobulin A; interleukin 17; neutralizing antibody; recombinant protein; water oil cream; emulsion; flagellin; hemagglutinin, avian influenza A virus; immunoglobulin A; immunological adjuvant; influenza vaccine; Influenza virus hemagglutinin; interleukin 17; recombinant protein; subunit vaccine; virus antibody; adjuvant therapy; animal cell; animal experiment; animal model; Article; B lymphocyte; cervical lymph node; comparative study; controlled study; cytokine production; enzyme linked immunosorbent assay; enzyme linked immunospot assay; female; influenza A (H5N1); influenza vaccination; Influenza virus A H5N1; liquid; lung lavage; mouse; nonhuman; priority journal; protein purification; Salmonella enterica serovar Typhimurium; spleen cell; T lymphocyte; animal; Bagg albino mouse; biosynthesis; blood; bronchoalveolar lavage fluid; chemistry; emulsion; genetics; immunology; Influenza A virus (H5N1); intranasal drug administration; mucosal immunity; nasal lavage fluid; Orthomyxoviridae Infections; secretion (process); T lymphocyte; virology; Adjuvants, Immunologic; Administration, Intranasal; Animals; Antibodies, Viral; B-Lymphocytes; Bronchoalveolar Lavage Fluid; Emulsions; Female; Flagellin; Hemagglutinin Glycoproteins, Influenza Virus; Immunity, Mucosal; Immunoglobulin A; Influenza A Virus, H5N1 Subtype; Influenza Vaccines; Interleukin-17; Mice, Inbred BALB C; Nasal Lavage Fluid; Orthomyxoviridae Infections; Recombinant Proteins; Salmonella typhimurium; T-Lymphocytes; Vaccines, Subunit |
Appears in Collections: | 醫學系 |
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