https://scholars.lib.ntu.edu.tw/handle/123456789/541997
標題: | Expression of human AR cDNA driven by its own promoter results in mild promotion, but not suppression, of growth in human prostate cancer PC-3 cells | 作者: | Altuwaijri S. Wu C.-C. Niu Y.-J. Mizokami A. HONG-CHIANG CHANG Chang C. |
關鍵字: | Androgen ablation therapy; Androgen receptor; Apoptosis; Proliferation; Prostate cancer | 公開日期: | 2007 | 卷: | 9 | 期: | 2 | 起(迄)頁: | 181-188 | 來源出版物: | Asian Journal of Andrology | 摘要: | Aim: To examine the physiological role of the androgen receptor (AR) in the PC-3 cell line by transfecting full-length functional AR cDNA driven by its natural human AR promoter. Methods: We generated an AR-expressing PC-3(AR)9 stable clone that expresses AR under the control of the natural human AR promoter and compared its proliferation to that of the PC-3(AR)2 (stable clone that expresses AR under the control of the cytomegalovirus (CMV) promoter, established by Heisler et al.) after androgen treatment. Results: We found that dihydrotestosterone (DHT) from 0.001 nmol/L to 10 nmol/L induces cell cycle arrest or inhibits proliferation of PC-3(AR)2 compared with its vector control, PC-3(pIRES). In contrast, PC-3(AR)9 cell growth slightly increased or did not change when treated with physiological concentrations of 1 nmol/L DHT. Conclusion: These data suggest that intracellular control of AR expression levels through the natural AR promoter might be needed for determining AR function in androgen-independent prostate cancer (AIPC) PC-3 cells. Unlike previous publications that showed DHT mediated suppression of PC-3 growth after transfection of viral promoter-driven AR overexpression, we report here that DHT-mediated PC-3 proliferation is slightly induced or does not change compared with its baseline after reintroducing AR expression driven by its own natural promoter, as shown in PC-3(AR)9 prostate cancer cells. ? 2007 Asian Journal of Andrology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-33947269319&doi=10.1111%2fj.1745-7262.2007.00258.x&partnerID=40&md5=72a88e5673f8d03123ea09c24d620a0e https://scholars.lib.ntu.edu.tw/handle/123456789/541997 |
ISSN: | 1008-682X | DOI: | 10.1111/j.1745-7262.2007.00258.x | SDG/關鍵字: | androgen; androgen receptor; androstanolone; complementary DNA; androgen independent prostate cancer cell; apoptosis; article; cancer cell; cell clone; cell cycle arrest; cell growth; cell proliferation; controlled study; Cytomegalovirus; human; human cell; male; promoter region; prostate cancer; protein expression; protein function; Cell Cycle; Cell Line, Tumor; Cell Proliferation; Dihydrotestosterone; DNA, Complementary; Humans; Male; Promoter Regions (Genetics); Prostatic Neoplasms; Receptors, Androgen; Transfection |
顯示於: | 醫學系 |
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