https://scholars.lib.ntu.edu.tw/handle/123456789/545687
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Chen Y.-B. | en_US |
dc.contributor.author | Lan Y.-W. | en_US |
dc.contributor.author | Chen L.-G. | en_US |
dc.contributor.author | Huang T.-T. | en_US |
dc.contributor.author | Choo K.-B. | en_US |
dc.contributor.author | Cheng W.T.K. | en_US |
dc.contributor.author | Lee, Hsuan-Shu | en_US |
dc.contributor.author | Chong K.-Y. | en_US |
dc.creator | Chen Y.-B.; Lan Y.-W.; Chen L.-G.; Huang T.-T.; Choo K.-B.; Cheng W.T.K.; Lee, Hsuan-Shu; Chong K.-Y. | - |
dc.date.accessioned | 2021-02-02T06:40:50Z | - |
dc.date.available | 2021-02-02T06:40:50Z | - |
dc.date.issued | 2015 | - |
dc.identifier.issn | 1355-8145 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84943356927&doi=10.1007%2fs12192-015-0627-7&partnerID=40&md5=ed5407b6fcb4368346f191ff5d1c8642 | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/545687 | - |
dc.description.abstract | Chronic obstructive pulmonary disease (COPD) is a sustained blockage of the airways due to lung inflammation occurring with chronic bronchitis and/or emphysema. Progression of emphysema may be slowed by vascular endothelial growth factor A (VEGFA), which reduces apoptotic tissue depletion. Previously, authors of the present report demonstrated that cis-resveratrol (c-RSV)-induced heat-shock protein 70 (HSP70) promoter-regulated VEGFA expression promoted neovascularization of genetically modified mesenchymal stem cells (HSP-VEGFA-MSC) in a mouse model of ischemic disease. Here, this same stem cell line was evaluated for its protective capacity to alleviate elastase-induced pulmonary emphysema in mice. Results of this study showed that c-RSV-treatment of HSP-VEGFA-MSC exhibited synergy between HSP70 transcription activity and induced expression of anti-oxidant-related genes when challenged by cigarette smoke extracts. Eight weeks after jugular vein injection of HSP-VEGFA-MSC into mice with elastase-induced pulmonary emphysema followed by c-RSV treatment to induce transgene expression, significant improvement was observed in respiratory functions. Expression of VEGFA, endogenous nuclear factor erythroid 2-related factor (Nrf 2), and manganese superoxide dismutase (MnSOD) was significantly increased in the lung tissues of the c-RSV-treated mice. Histopathologic examination of treated mice revealed gradual but significant abatement of emphysema and restoration of airspace volume. In conclusion, the present investigation demonstrates that c-RSV-regulated VEGFA expression in HSP-VEGFA-MSC significantly improved the therapeutic effects on the treatment of COPD in the mouse, possibly avoiding side effects associated with constitutive VEGFA expression. ? 2015, Cell Stress Society International. | - |
dc.publisher | Cell Stress and Chaperones | - |
dc.relation.ispartof | Cell Stress and Chaperones | - |
dc.subject | Emphysema; Hsp70 promoter; Mouse model; Resveratrol; Stem cell-based gene therapy; VEGFA | - |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | cigarette smoke; elastase; heat shock protein 70; lentivirus vector; manganese superoxide dismutase; resveratrol; transcription factor Nrf2; vasculotropin A; heat shock protein 70; pancreatic elastase; resveratrol; smoke; stilbene derivative; vasculotropin A; VEGFA protein, human; animal cell; animal experiment; animal model; antioxidant responsive element; Article; bone marrow derived mesenchymal stem cell; cell survival; elastase-induced emphysema; female; jugular vein; lung function; mouse; mouse model; nonhuman; nonviral gene delivery system; nucleotide sequence; oxidative stress; priority journal; promoter region; protein expression; quantitative analysis; real time polymerase chain reaction; respiratory function; Western blotting; adverse effects; animal; C57BL mouse; cell culture; disease model; drug effects; emphysema; genetics; lung; mesenchymal stroma cell; metabolism; smoke; tobacco; Animals; Cell Survival; Cells, Cultured; Disease Models, Animal; Emphysema; Female; HSP70 Heat-Shock Proteins; Lung; Mesenchymal Stromal Cells; Mice; Mice, Inbred C57BL; Pancreatic Elastase; Smoke; Stilbenes; Tobacco; Vascular Endothelial Growth Factor A | - |
dc.title | Mesenchymal stem cell-based HSP70 promoter-driven VEGFA induction by resveratrol alleviates elastase-induced emphysema in a mouse model | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1007/s12192-015-0627-7 | - |
dc.identifier.pmid | 26243699 | - |
dc.identifier.scopus | 2-s2.0-84943356927 | - |
dc.relation.pages | 979-989 | - |
dc.relation.journalvolume | 20 | - |
dc.relation.journalissue | 6 | - |
item.cerifentitytype | Publications | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Biotechnology | - |
crisitem.author.orcid | 0000-0002-8017-0434 | - |
crisitem.author.parentorg | College of Bioresources and Agriculture | - |
顯示於: | 醫學系 |
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