https://scholars.lib.ntu.edu.tw/handle/123456789/545884
標題: | Inhibitory effect and mechanisms of an anthocyanins- and anthocyanidins-rich extract from purple-shoot tea on colorectal carcinoma cell proliferation | 作者: | Hsu C.-P. Shih Y.-T. BOR-RU LIN Chiu C.-F. Lin C.-C. |
關鍵字: | apoptosis; cell cycle; colorectal carcinoma; purple-shoot tea | 公開日期: | 2012 | 卷: | 60 | 期: | 14 | 起(迄)頁: | 3686-3692 | 來源出版物: | Journal of Agricultural and Food Chemistry | 摘要: | One newly bred variety of tea cultivar, purple-shoot tea, was selected to evaluate its antiproliferative effects on colorectal carcinoma cells, as well as normal colon cells. The phytochemicals and identified catechins of purple-shoot tea extract (PTE) were significantly higher than that of ordinary tea, especially the anthocyanins (surpassed by 135-fold) and anthocyanidins (surpassed by 3.5-fold). PTE inhibited the proliferation of COLO 320DM (IC 50 = 64.9 μg/mL) and HT-29 (IC 50 = 55.2 μg/mL) by blocking cell cycle progression during the G 0/G 1 phase and inducing apoptotic death. Western blotting indicated that PTE induced cell cycle arrest by reducing the expression of cyclin E and cyclin D1 in COLO 320DM and the upregulation of p21 and p27 cyclin-dependent kinase inhibitors in HT-29. Two cells treated with PTE also indicated the cleavage of PARP, activation of caspase 3, and an increased Bax/Bcl-2 ratio. Our results showed that PTE is a potential novel dietary agent for colorectal cancer chemoprevention. ? 2012 American Chemical Society. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84859567147&doi=10.1021%2fjf204619n&partnerID=40&md5=a4d82d7ebe8a3500cf9635bd49f25a5f https://scholars.lib.ntu.edu.tw/handle/123456789/545884 |
ISSN: | 0021-8561 | DOI: | 10.1021/jf204619n | SDG/關鍵字: | Anthocyanidins; Antiproliferative effect; Apoptotic; Caspase-3; cell cycle; Cell-cycle arrest; Cell-cycle progression; Colon cells; Colorectal cancer; colorectal carcinoma; Cyclin E; Cyclin-dependent kinase inhibitors; CyclinD1; Inhibitory effect; Phytochemicals; purple-shoot tea; Tea cultivars; Tea extracts; Up-regulation; Western blotting; Cell death; Cell proliferation; Phenols; Anthocyanins; anthocyanin; flavonoid; plant extract; animal; apoptosis; article; cell cycle; cell line; cell proliferation; cell strain HT29; chemistry; colon tumor; colorectal tumor; drug effect; epithelium cell; human; pathology; rat; tea; tumor cell line; Animals; Anthocyanins; Apoptosis; Cell Cycle; Cell Line; Cell Line, Tumor; Cell Proliferation; Colonic Neoplasms; Colorectal Neoplasms; Epithelial Cells; Flavonoids; HT29 Cells; Humans; Plant Extracts; Rats; Tea |
顯示於: | 醫學系 |
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