https://scholars.lib.ntu.edu.tw/handle/123456789/547041
標題: | Mesenchymal stem cells from a hypoxic culture improve nerve regeneration | 作者: | Wang, J.-P. Liao, Y.-T. Wu, S.-H. Chiang, E.-R. Hsu, S.-H. Tseng, T.-C. Hung, S.-C. SHAN-HUI HSU |
關鍵字: | animal model; bone marrow-derived mesenchymal stem cells (BMSCs); hypoxia; nerve regeneration; neuronal differentiation; sciatic nerve defect | 公開日期: | 2020 | 來源出版物: | Journal of Tissue Engineering and Regenerative Medicine | 摘要: | Repairing the peripheral nerves following a segmental defect injury remains surgically challenging. Because of some disadvantages of nerve grafts, nerve regeneration, such as conduits combined with bone marrow-derived mesenchymal stem cells (BMSCs), may serve as an alternative. BMSCs expand under hypoxic conditions, decrease in senescence, and increase in proliferation and differentiation potential into the bone, fat, and cartilage. The purpose of this study was to investigate whether BMSCs increased the neuronal differentiation potential following expansion under hypoxic conditions. Isolated human BMSCs (hBMSCs) expand under hypoxia or normoxia, and neuronal differentiation proceeds under normoxia. in vitro tests revealed hypoxia culture enhanced the RNA and protein expression of neuronal markers. The electrophysiology of hBMSC-differentiated neuron-like cells was also enhanced by the hypoxia culturing. Our animal model indicated that the potential treatment of hypoxic rat BMSCs (rBMSCs) was better than that of normoxic rBMSCs because the conduit with the hypoxic rBMSCs injection demonstrated the highest recovery rate of gastrocnemius muscle weights. There were more toluidine blue-stained myelinated nerve fibers in the hypoxic rBMSCs group than in the normoxic group. To sum up, BMSCs cultured under hypoxia increased the potential of neuronal differentiation both in vivo and in vitro. ? 2020 John Wiley & Sons, Ltd. |
URI: | https://www.scopus.com/inward/record.url?eid=2-s2.0-85092396758&partnerID=40&md5=7ccecc2356aedbb822f103aff41e4e81 https://scholars.lib.ntu.edu.tw/handle/123456789/547041 |
DOI: | 10.1002/term.3136 | SDG/關鍵字: | Animals; Bone; Cell culture; Electrophysiology; Muscle; Neurons; Stem cells; Animal model; Bone marrow-derived mesenchymal stem cell; Bone marrow-derived mesenchymal stem cells; Bone-marrow-derived mesenchymal stem cells; Hypoxia; Hypoxic condition; Nerve regeneration; Neuronal differentiation; Sciatic nerve defect; Sciatic nerves; Defects; RNA; adult; animal cell; animal experiment; animal model; animal tissue; Article; bone marrow derived mesenchymal stem cell; cell culture; cell isolation; cohort analysis; controlled study; electrophysiology; gastrocnemius muscle; human; human cell; hypoxia; in vitro study; in vivo study; mesenchymal stem cell transplantation; muscle weight; myelinated nerve; nerve cell; nerve cell differentiation; nerve regeneration; nonhuman; priority journal; protein expression; rat; sciatic nerve injury; Sprague Dawley rat; stem cell expansion; animal; cell culture; cell differentiation; cell hypoxia; cell membrane; cell shape; cytology; disease model; gene expression regulation; injury; mesenchymal stem cell; metabolism; nerve regeneration; pathology; physiology; sciatic nerve; Animals; Cell Differentiation; Cell Hypoxia; Cell Membrane; Cell Shape; Cells, Cultured; Disease Models, Animal; Gene Expression Regulation; Humans; Mesenchymal Stem Cell Transplantation; Mesenchymal Stem Cells; Nerve Regeneration; Neurons; Rats, Sprague-Dawley; Sciatic Nerve |
顯示於: | 高分子科學與工程學研究所 |
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