https://scholars.lib.ntu.edu.tw/handle/123456789/548561
標題: | Developmental pattern of the neuronal intermediate filament inaa in the zebrafish retina | 作者: | MENG-LIN LIAO Peng, Wei-Hau Kan, Daphne CHUNG-LIANG CHIEN |
關鍵字: | development; neurofilament; retina; zebrafish (RRID:ZIRC_ZL1); α-internexin | 公開日期: | 2016 | 出版社: | WILEY-BLACKWELL | 卷: | 524 | 期: | 18 | 起(迄)頁: | 3810 | 來源出版物: | The Journal of comparative neurology | 摘要: | α-Internexin is a member of the neuronal intermediate filament (nIF) protein family, which also includes peripherin and neurofilament (NF) triplet proteins. Previous studies found that expression of α-internexin precedes that of the NF triplet proteins in mammals and suggested that α-internexin plays a key role in the neuronal cytoskeleton network during development. In this study, we aimed to analyze the expression patterns and function of internexin neuronal intermediate filament protein-alpha a (inaa), the encoding gene of which is a homolog of the mammalian α-internexin, during retinal development in zebrafish. Via in vitro and in vivo studies, we demonstrated that zebrafish inaa is an α-internexin homolog that shares characteristics with nIFs. An immunohistochemical analysis of zebrafish revealed that inaa was distributed dynamically in the developing retina. It was widely localized in retinal neuroepithelial cells at 1 day postfertilization (dpf), and was mainly found in the ganglion cell layer (GCL) and inner part of the inner nuclear layer (INL) from 3-9 dpf; after 14 dpf, it was restricted to the outer nuclear layer (ONL). Moreover, we demonstrated for the first time that inaa acted distinctively from the cytoskeletal scaffold of zebrafish cone photoreceptors during development. In conclusion, we demonstrated the morphological features of a novel nIF, inaa, and illustrated its developmental expression pattern in the zebrafish retina. J. Comp. Neurol. 524:3810-3826, 2016. © 2016 Wiley Periodicals, Inc. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/548561 | ISSN: | 0021-9967 | DOI: | 10.1002/cne.24033 |
顯示於: | 解剖學暨細胞生物學科所 |
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