https://scholars.lib.ntu.edu.tw/handle/123456789/549054
標題: | Integrative analyses of noncoding RNAs reveal the potential mechanisms augmenting tumor malignancy in lung adenocarcinoma | 作者: | Shih, J.-H. Chen, H.-Y. Lin, S.-C. Yeh, Y.-C. Shen, R. Lang, Y.-D. Wu, D.-C. CHIEN-YU CHEN Chen, R.-H. Chou, T.-Y. Jou, Y.-S. |
公開日期: | 2020 | 卷: | 48 | 期: | 3 | 起(迄)頁: | 1175-1191 | 來源出版物: | Nucleic acids research | 摘要: | Precise noncoding RNA (ncRNA)-based network prediction is necessary to reveal ncRNA functions and pathological mechanisms. Here, we established a systemic pipeline to identify prognostic ncRNAs, predict their functions and explore their pathological mechanisms in lung adenocarcinoma (LUAD). After in silico and experimental validation based on evaluations of prognostic value in multiple LUAD cohorts, we selected the PTTG3P pseudogene from among other prognostic ncRNAs (MIR497HG, HSP078, TBX5-AS1, LOC100506990?and C14orf64) for mechanistic studies. PTTG3P upregulation in LUAD cells shortens the metaphase to anaphase transition in mitosis, increases cell viability after cisplatin or paclitaxel treatment, facilitates tumor growth that leads to poor survival in orthotopic lung models, and is associated with a poor survival rate in LUAD patients in the TCGA cohort who received chemotherapy. Mechanistically, PTTG3P acts as an ncRNA that interacts with the transcription factor FOXM1 to regulate the transcriptional activation of the mitotic checkpoint kinase BUB1B, which augments tumor growth and chemoresistance and leads to poor outcomes for LUAD patients. Overall, we established a systematic strategy to uncover prognostic ncRNAs with functional prediction methods suitable for pan-cancer studies. Moreover, we revealed that PTTG3P, due to its upregulation of the PTTG3P/FOXM1/BUB1B axis, could be a therapeutic target for LUAD patients. ? The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. |
URI: | https://www.scopus.com/inward/record.url?eid=2-s2.0-85082059180&partnerID=40&md5=bc3589274a78acb253490a5c50e90a5f https://scholars.lib.ntu.edu.tw/handle/123456789/549054 |
DOI: | 10.1093/nar/gkz1149 | SDG/關鍵字: | BUB1B protein, human; cell cycle protein; forkhead box protein M1; protein serine threonine kinase; untranslated RNA; adenocarcinoma; animal; cell proliferation; chromatin; computer simulation; drug resistance; genetics; human; lung tumor; male; metabolism; mitosis; mortality; mouse; pathology; prognosis; signal transduction; tumor cell line; Adenocarcinoma; Animals; Cell Cycle Proteins; Cell Line, Tumor; Cell Proliferation; Chromatin; Computer Simulation; Drug Resistance, Neoplasm; Forkhead Box Protein M1; Humans; Lung Neoplasms; Male; Mice; Mitosis; Prognosis; Protein-Serine-Threonine Kinases; RNA, Untranslated; Signal Transduction |
顯示於: | 生物機電工程學系 |
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