https://scholars.lib.ntu.edu.tw/handle/123456789/550774
標題: | Updated phylogenetic analysis of the spike gene and identification of a novel recombinant porcine epidemic diarrhoea virus strain in Taiwan | 作者: | Hsueh, F.-C. Lin, C.-N. Chiou, H.-Y. Chia, M.-Y. Chiou, M.-T. Haga, T. Kao, C.-F. Chang, C.Y. CHIAN-REN JENG YEN-CHEN CHANG HUI-WEN CHANG |
公開日期: | 2020 | 卷: | 67 | 期: | 1 | 起(迄)頁: | 417-430 | 來源出版物: | Transboundary and Emerging Diseases | 摘要: | New variants of porcine epidemic diarrhoea virus (PEDV) causing a highly contagious intestinal disease, porcine epidemic diarrhoea virus (PED), have resulted in high mortality in suckling pigs across several countries since 2013. After 2015, the prevalence of the genogroup 2b (G2b) PEDVs decreased in a cyclical pattern with endemic seasonal outbreaks occasionally seen. To better understand the genetic diversity of PEDVs recently circulating in Taiwan, full-length spike (S) genes of 31 PEDV strains from 28 pig farms collected during 2016–2018 were sequenced. While the majority of S gene sequences (from 27/28 farms) were closely related to the previous G2b PEDV strains, increased genetic diversities leading to several nonsynonymous mutations scattering in the neutralizing epitopes of the S gene were detected in PEDVs recently circulating in Taiwan. Furthermore, novel recombinant variants, the PEDV TW/Yunlin550/2018 strains exhibiting recombinant events between a previously isolated Taiwan PEDV G2b strain and a wild-type PEDV G1a strain, were identified and further classified into a new genogroup, G1c. These results provide updated information about the genetic diversity of currently circulating PEDVs in the field and could help to develop more suitable strategies for controlling this disease. ? 2019 Blackwell Verlag GmbH |
URI: | https://www.scopus.com/inward/record.url?eid=2-s2.0-85073927493&partnerID=40&md5=045c31b9a713dea87e77ac9386cb4043 https://scholars.lib.ntu.edu.tw/handle/123456789/550774 |
DOI: | 10.1111/tbed.13365 | SDG/關鍵字: | epitope; neutralizing antibody; nonstructural protein 2; virus RNA; virus spike protein; coronavirus spike glycoprotein; amino acid analysis; Article; controlled study; feces analysis; gene; gene sequence; genetic variability; high throughput sequencing; next generation sequencing; nonhuman; nucleotide sequence; phylogenetic tree; phylogeny; pig; porcine epidemic diarrhea; Porcine epidemic diarrhea virus; prevalence; recombinant porcine epidemic diarrhoea virus; reverse transcription polymerase chain reaction; RNA extraction; sequence alignment; sequence analysis; sequence homology; spike gene; Taiwan; ultraviolet spectrophotometry; virus identification; virus isolation; virus recombinant; virus spike; agricultural land; animal; Coronavirus infection; epidemic; female; genetic variation; genetics; genotype; isolation and purification; phylogeny; swine disease; veterinary medicine; virology; Animals; Coronavirus Infections; Disease Outbreaks; Farms; Female; Genetic Variation; Genotype; Phylogeny; Porcine epidemic diarrhea virus; Spike Glycoprotein, Coronavirus; Swine; Swine Diseases; Taiwan |
顯示於: | 分子暨比較病理生物學研究所 |
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