https://scholars.lib.ntu.edu.tw/handle/123456789/550983
標題: | Prevention of dextran sulfate sodium-induced mouse colitis by the fungal protein Ling Zhi-8 via promoting the barrier function of intestinal epithelial cells | 作者: | Chen, Yu-Huan Shin, Jenn-Yeu Wei, Hsiu-Mei Lin, Chi-Chien LINDA CHIA-HUI YU Liao, Wei-Ting Chen, Dz-Chi CHING-LIANG CHU |
公開日期: | 1-三月-2021 | 卷: | 12 | 期: | 4 | 來源出版物: | Food & function | 摘要: | The fungal immunomodulatory protein Ling Zhi-8 (LZ-8) isolated from Ganoderma lucidum (GL) regulates immune cells and inhibits tumor growth; however, the role of LZ-8 in protecting intestinal epithelial cells (IECs) is unknown. In this study, we aim to investigate the functional effect of LZ-8 on IECs. LZ-8 effectively rescued the pro-inflammatory cytokine-induced loss of tight junctions (TJs) by enhancing transepithelial electrical resistance (TEER), reducing permeability, and maintaining the distribution of TJ proteins, in Caco-2 cells. Mechanistically, LZ-8 blocked the upregulation of myosin light chain kinase (MLCK) and NF-kB activation by TLR2-mediated suppression of cytokine signaling (SOCS)-1 expression. Furthermore, LZ-8 pre-treatment reduced the pathological scores of dextran sulfate sodium (DSS)-induced colitis in mice. These results indicated that LZ-8 protected the barrier function of IECs against inflammation. Thus, LZ-8 may potentially be a novel candidate for treating inflammatory bowel disease (IBD). |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/550983 | DOI: | 10.1039/d0fo02604b | SDG/關鍵字: | Cytology; Dextran; Diseases; Mammals; Proteins; Sodium; Sulfur compounds; Barrier functions; Cytokine signaling; Dextran sulfate sodium; Dextran sulfate sodium (DSS) induced colitis; Inflammatory bowel disease; Intestinal epithelial cells; Myosin light ch |
顯示於: | 生理學科所 |
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