https://scholars.lib.ntu.edu.tw/handle/123456789/551365
標題: | Maternal hypercholesterolemia exacerbates atherosclerosis lesions in female offspring through potentiating macrophage polarization toward an inflammatory M1 phenotype | 作者: | Chen, Sin-Yu Chen, Yi-Zhen Lee, Yi-Jing Jiang, Chung-Lin SHAO-CHUN LU FU-JUNG LIN |
關鍵字: | Apolipoprotein E; Atherosclerosis; Hypercholesterolemia; Inflammation; Macrophage | 公開日期: | 31-十二月-2020 | 卷: | 90 | 來源出版物: | The Journal of nutritional biochemistry | 摘要: | Maternal hypercholesterolemia induces early onset of cardiovascular diseases in offspring; however, its underlying mechanism remains poorly understood. We hypothesized that maternal hypercholesterolemia increases offspring susceptibility to atherosclerosis in adulthood through developmental modifications of macrophages. Female apolipoprotein E (ApoE)-deficient mice were fed a Western-type diet (WD) or a control diet (CD) prior to and throughout gestation and lactation. The offspring were all fed a WD after weaning. Sixteen-week-old female offspring of WD-fed dams showed a significant increase in atherosclerotic lesions of the aorta and aortic root compared with those of CD-fed dams. This effect was associated with increased macrophage accumulation within lesions, macrophage inflammation and an increase in circulating Ly6Chigh monocyte and F4/80 macrophage counts. We further evidenced that in utero WD exposure promoted macrophage polarization toward the M1 phenotype by elevating M1 markers (Cd86, Inos/Nos2) without affecting M2 markers (Cd206, Arg1). Proinflammatory cytokine synthesis was also enhanced in response to LPS. Finally, maternal WD intake strongly inhibited the macrophage expression of Pparg and Lxra, which was associated with aberrant DNA methylation of Lxra promoter. Our findings demonstrate that maternal hypercholesterolemia exacerbates atherosclerosis, in part by altering the epigenetic state of the macrophage genome of the offspring, imprinting gene expression, and changing macrophage polarization, which ultimately contributes to plaque macrophage burden. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/551365 | ISSN: | 09552863 | DOI: | 10.1016/j.jnutbio.2020.108575 | SDG/關鍵字: | ABC transporter A1; ABC transporter G1; apolipoprotein E; arginase 1; cd206 antigen; CD86 antigen; cell adhesion molecule 1; chemokine receptor CX3CR1; cholesterol; fatty acid; fractalkine; genomic DNA; glucose; high density lipoprotein cholesterol; induc |
顯示於: | 生物化學暨分子生物學科研究所 |
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