https://scholars.lib.ntu.edu.tw/handle/123456789/552638
標題: | Trichoderma reesei Rad51 tolerates mismatches in hybrid meiosis with diverse genome sequences | 作者: | Li, Wan Chen Lee, Chia Yi Lan, Wei Hsuan Woo, Tai Ting Liu, Hou Cheng Yeh, Hsin Yi Chang, Hao Yen Chuang, Yu Chien Chen, Chiung Ya Chuang, Chi Ning Chen, Chia Ling Hsueh, Yi Ping HUNG-WEN LI Chi, Peter Wang, Ting Fang |
關鍵字: | Dmc1 | Homologous recombination | Meiosis | Rad51 | Trichoderma | 公開日期: | 23-二月-2021 | 出版社: | National Academy of Sciences | 卷: | 118 | 期: | 8 | 起(迄)頁: | e2007192118 | 來源出版物: | Proceedings of the National Academy of Sciences of the United States of America | 摘要: | © 2021 National Academy of Sciences. All rights reserved. Most eukaryotes possess two RecA-like recombinases (ubiquitous Rad51 and meiosis-specific Dmc1) to promote interhomolog recombination during meiosis. However, some eukaryotes have lost Dmc1. Given that mammalian and yeast Saccharomyces cerevisiae (Sc) Dmc1 have been shown to stabilize recombination intermediates containing mismatches better than Rad51, we used the Pezizomycotina filamentous fungus Trichoderma reesei to address if and how Rad51-only eukaryotes conduct interhomolog recombination in zygotes with high sequence heterogeneity. We applied multidisciplinary approaches (next- and third-generation sequencing technology, genetics, cytology, bioinformatics, biochemistry, and single-molecule biophysics) to show that T. reesei Rad51 (TrRad51) is indispensable for interhomolog recombination during meiosis and, like ScDmc1, TrRad51 possesses better mismatch tolerance than ScRad51 during homologous recombination. Our results also indicate that the ancestral TrRad51 evolved to acquire ScDmc1-like properties by creating multiple structural variations, including via amino acid residues in the L1 and L2 DNA-binding loops. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/552638 | ISSN: | 00278424 1091-6490 |
DOI: | 10.1073/pnas.2007192118 |
顯示於: | 生化科學研究所 |
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