https://scholars.lib.ntu.edu.tw/handle/123456789/557537
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Ou-Yang P. | en_US |
dc.contributor.author | BOR-LUEN CHIANG | en_US |
dc.contributor.author | Hwang L.-H. | en_US |
dc.contributor.author | Chen Y.-G. | en_US |
dc.contributor.author | PEI-MING YANG | en_US |
dc.contributor.author | Chi W.-K. | en_US |
dc.contributor.author | PEI-JER CHEN | en_US |
dc.contributor.author | DING-SHINN CHEN | en_US |
dc.date.accessioned | 2021-04-22T06:56:57Z | - |
dc.date.available | 2021-04-22T06:56:57Z | - |
dc.date.issued | 1999 | - |
dc.identifier.issn | 0146-6615 | - |
dc.identifier.uri | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84984567059&doi=10.1002%2f%28SICI%291096-9071%28199904%2957%3a4%3c345%3a%3aAID-JMV3%3e3.0.CO%3b2-N&partnerID=40&md5=c8c127eebc52e5b421b9b3afc676d39e | - |
dc.identifier.uri | https://scholars.lib.ntu.edu.tw/handle/123456789/557537 | - |
dc.description.abstract | The nonstructural (NS3) region protein of hepatitis C virus (HCV) possesses major B-cell epitopes that induce antibodies after infection. To elucidate further the characteristics of these B cells and their role in the immune regulation of HCV infection, T9 (portion of NS3 region, amino acids [a.a.] 1188-1493)-specific monoclonal antibodies were derived and mapped for B-cell antigenic determinants with recombinant proteins. A total of 10 T9- specific hybridomas were generated and tested for B-cell antigenic determinants. To analyze the B-cell antigenic determinants, eight recombinant proteins including NS3-e (a.a. 1175-1334), NS3-a' (a.a. 1175-1250), NS3-a (a.a. 1251-1334), NS3-b (a.a. 1323-1412), NS3-c (a.a. 1407-1499), NS3-a/b (a.a. 1251-1412), NS3-bc (a.a. 1323-1499), and NS3-abc (a.a. 1251-1499) encoded by NS3-region internal clones were expressed and tested for immunoblotting. The data suggested IgG hybridomas recognized NS3-a, NS3-a', or NS3-b protein by immunoblotting. By contrast, the NS3-e protein bears the major antigenic determinant recognized by human sera. Half of the hybridomas were found to react with protein NS3-a', which is not a major B-cell antigenic determinant in humans. These data suggested that conformational epitopes in vivo may be important for B-cell recognition. | en_US |
dc.publisher | Wiley-Liss Inc. | en_US |
dc.relation.ispartof | Journal of Medical Virology | en_US |
dc.subject.classification | [SDGs]SDG3 | - |
dc.subject.other | B lymphocyte antigen; epitope; immunoglobulin G; monoclonal antibody; recombinant protein; virus protein; antigen recognition; article; controlled study; Hepatitis C virus; human; human cell; hybridoma; immunoblotting; immunoregulation; peptide mapping; Hepatitis C virus; RNA viruses | - |
dc.title | Characterization of monoclonal antibodies against hepatitis C virus nonstructural protein 3: Different antigenic determinants from human B cells | en_US |
dc.type | journal article | en |
dc.identifier.doi | 10.1002/(SICI)1096-9071(199904)57:4<345 | - |
dc.identifier.pmid | 10089044 | - |
dc.identifier.scopus | 2-s2.0-84984567059 | - |
dc.relation.pages | 345-350 | en_US |
dc.relation.journalvolume | 57 | en_US |
dc.relation.journalissue | 4 | en_US |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.cerifentitytype | Publications | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
crisitem.author.dept | Pediatrics | - |
crisitem.author.dept | Pediatrics-NTUH | - |
crisitem.author.dept | Clinical Medicine | - |
crisitem.author.dept | Oral Biology | - |
crisitem.author.dept | College of Life Science | - |
crisitem.author.dept | Immunology | - |
crisitem.author.dept | Internal Medicine | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.dept | Clinical Medicine | - |
crisitem.author.dept | Internal Medicine-NTUH | - |
crisitem.author.orcid | 0000-0002-6705-0286 | - |
crisitem.author.orcid | 0000-0003-3473-4611 | - |
crisitem.author.orcid | 0000-0001-8316-3785 | - |
crisitem.author.orcid | 0000-0001-7791-6154 | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
crisitem.author.parentorg | College of Medicine | - |
crisitem.author.parentorg | National Taiwan University Hospital | - |
顯示於: | 醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。