https://scholars.lib.ntu.edu.tw/handle/123456789/557570
標題: | Detection of divergent hepatitis C virus envelope sequences | 作者: | JIA-HORNG KAO PEI-JER CHEN Lai M.-Y. PEI-MING YANG JIN-CHUAN SHEU Wang T.-H. DING-SHINN CHEN |
公開日期: | 1994 | 出版社: | Kluwer Academic Publishers | 卷: | 1 | 期: | 3 | 起(迄)頁: | 158-162 | 來源出版物: | Journal of Biomedical Science | 摘要: | The nucleotide sequences of the putative envelope region (E1) and the junction between the E1 and envelope 2/nonstructural 1 (E2/NS1) region of the hepatitis C virus (HCV) genome are divergent among different genotypes. To characterize them, we introduced a set of nested primers that are conserved among four different genotypes (types I-IV) of HCV for polymerase chain reaction (PCR) amplification. The amplified products include the variable full-length E1 region, and the 5′ end of the E2/NS1 region, the so-called hypervariable region-1 (HVR-1). Of 53 patients with histologically confirmed chronic liver disease and HCV viremia, type II virus was the most dominant strain as detected by the PCR genotyping method and the envelope region could be amplified in more than half of them irrespective of their genotypes. The specificity was confirmed by subsequent nucleotide sequence analysis. The positivity of envelope region PCR was not correlated with histologic diagnosis and hepatitis activities in these patients. Our results suggest that the nested primers can amplify the variable E1 and hypervariable 5′ end of E2/NS1 of the HCV genome with moderate efficiency, and thus will be useful in future studies of HCV infections. ? 1994 National Science Council. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84984552569&doi=10.1007%2fBF02253343&partnerID=40&md5=574aa6442577eabe8ee9fc3c4670dc93 https://scholars.lib.ntu.edu.tw/handle/123456789/557570 |
ISSN: | 1021-7770 | DOI: | 10.1007/BF02253343 |
顯示於: | 醫學系 |
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