https://scholars.lib.ntu.edu.tw/handle/123456789/558397
Title: | Prominin-1-Specific Binding Peptide-Modified Apoferritin Nanoparticle Carrying Irinotecan as a Novel Radiosensitizer for Colorectal Cancer Stem-Like Cells | Authors: | JENNY LING-YU CHEN Tsai Y.-C. Tsai M.-H. Lee S.-Y. Wei M.-F. SUNG-HSIN KUO MING-JIUM SHIEH |
Issue Date: | 2017 | Journal Volume: | 34 | Journal Issue: | 5 | Source: | Particle and Particle Systems Characterization | Abstract: | Resistance of cancer stem cells to radiotherapy remains a major obstacle to successful cancer management. Prominin-1 (PROM1) is a cancer stem cell marker. Nanoparticle (NP) chemotherapeutics preferentially accumulate in tumors and are able to target cancer and cancer stem-like cells through cancer cell-specific ligands, making them uniquely suited as radiosensitizers for chemoradiation therapy. Using a biocompatible apoferritin NP, a PROM1-targeted NP carrying irinotecan (PROM1-NP) is engineered. The synergistic effect of the NP and irradiation is evaluated in PROM1-overexpressing HCT-116 colorectal cancer cell lines in vitro and in vivo. PROM1-NP has a size of 17.2 ± 0.2 nm and surface charge of ?13.5 ± 0.2 mV. It demonstrates higher intracellular uptake than nontargeted NP or irinotecan alone. Treatment with PROM1-NPs decreases HCT-116 cell proliferation in a dose- and time-dependent manner. In vitro radiosensitization reveals that PROM1-NP is significantly more effective as a radiosensitizer than nontargeted NP or irinotecan. HCT-116 tumor xenograft growth is markedly slower following treatment with PROM1-NP plus irradiation, suggesting that PROM1-NP is more effective as a radiosensitizer than irinotecan and nontargeted NP in vivo. This study provides the first preclinical evidence of the effectiveness of PROM1-targeted NP formulation of irinotecan as a radiosensitizer. ? 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/558397 | ISSN: | 0934-0866 | DOI: | 10.1002/ppsc.201600424 | SDG/Keyword: | Biocompatibility; Cell culture; Cell proliferation; Cells; Cytology; Irradiation; Nanoparticles; Stem cells; Tumors; Apoferritin; Chemoradiation therapies; Colorectal cancer; Colorectal cancer cell; Intracellular uptake; Irinotecan; Radio-sensitization; Synergistic effect; Diseases |
Appears in Collections: | 醫學院附設醫院 (臺大醫院) |
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