https://scholars.lib.ntu.edu.tw/handle/123456789/560820
標題: | Capsular polysaccharide synthesis regions in Klebsiella pneumoniae serotype K57 and a new capsular serotype | 作者: | Pan Y.-J. Fang H.-C. Yang H.-C. Lin T.-L. Hsieh P.-F. FENG-CHIAO TSAI Keynan Y. JIN-TOWN WANG |
公開日期: | 2008 | 卷: | 46 | 期: | 7 | 起(迄)頁: | 2231-2240 | 來源出版物: | Journal of Clinical Microbiology | 摘要: | Community-acquired pyogenic liver abscess caused by Klebsiella pneumoniae is an emerging infectious disease. We explored the capsular polysaccharide synthesis (cps) regions of three non-K1, non-K2 K. pneumoniae strains, A1142, A7754, and A1517, from Taiwanese patients experiencing pyogenic liver abscess. Two of the strains, A1142 and A7754, belonged to capsular serotype K57, while the third belonged to a new capsular serotype, different from the previously reported 77 serotypes. Deletion and complementation experiments suggested that a unique K57 gene, a homologue of wzy, was essential for K57 capsular synthesis and confirmed that this gene cluster was a genetic coding region for K57. Compared to K1 and K2 strains, the three strains were all serum sensitive, suggesting that host factors might also be involved in the three patients. PCR using primers from specific genes for K57 was more sensitive and specific than traditional serotyping. The remaining strain, A1517, did not react to the antisera from any of the 77 serotypes, and none of the 77 reference strains reacted to the serum against this strain. Moreover, PCR analyses using various primer pairs from the serotype-specific open reading frames did not reveal cross-reactivity to any of the 77 reference strains, suggesting that this strain likely represents a new capsular type. We conclude that sequences from these two cps regions are very useful in detecting K57 and the new cps genotype. Copyright ? 2008, American Society for Microbiology. All Rights Reserved. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/560820 | ISSN: | 951137 | DOI: | 10.1128/JCM.01716-07 | SDG/關鍵字: | bacterial polysaccharide; bacterial DNA; bacterial polysaccharide; primer DNA; article; bacterial gene; bacterial membrane; bacterial strain; controlled study; cps gene; cross reaction; gene cluster; gene deletion; gene sequence; genetic complementation; genotype; human; K57 gene; Klebsiella pneumoniae; nonhuman; nucleotide sequence; open reading frame; polymerase chain reaction; priority journal; pyogenic liver abscess; sensitivity and specificity; serotype; Taiwan; wzy gene; classification; communicable disease; DNA sequence; Enterobacter infection; gene order; genetics; immunoblotting; isolation and purification; liver abscess; methodology; microbiology; molecular genetics; multigene family; restriction fragment length polymorphism; serotyping; synteny; Klebsiella pneumoniae; Bacterial Capsules; Community-Acquired Infections; DNA Primers; DNA, Bacterial; Gene Deletion; Gene Order; Genes, Bacterial; Genetic Complementation Test; Humans; Immunoblotting; Klebsiella Infections; Klebsiella pneumoniae; Liver Abscess; Molecular Sequence Data; Multigene Family; Open Reading Frames; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Polysaccharides, Bacterial; Sensitivity and Specificity; Sequence Analysis, DNA; Serotyping; Synteny; Taiwan |
顯示於: | 藥理學科所 |
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