https://scholars.lib.ntu.edu.tw/handle/123456789/561119
標題: | Diagnostic performance of an enzyme-linked immunospot assay for interferon-γ in skeletal tuberculosis | 作者: | Lai C.-C. Tan C.-K. Liu W.-L. Lin S.-H. YU-TSUNG HUANG Liao C.-H. PO-REN HSUEH |
公開日期: | 2011 | 卷: | 30 | 期: | 6 | 起(迄)頁: | 767-771 | 來源出版物: | European Journal of Clinical Microbiology and Infectious Diseases | 摘要: | The aim of this study was to evaluate the diagnostic performance of an enzyme-linked immunospot (ELISPOT) assay for interferon-γ in patients with suspected skeletal tuberculosis (TB). From March 2007 to June 2010, a total of 36 patients with suspected skeletal TB in a tertiary care hospital in Taiwan were enrolled. Twelve patients (35.3%) had culture-confirmed TB, three (8.8%) patients had probable TB, and the remaining 21 (58.3%) patients did not have TB. Fourteen patients with mycobacterial infection had available biopsy or surgical specimens for histopathological examination and 12 (85.7%) specimens had pathological features consistent with mycobacterial infection. Among the 12 patients with positive findings indicating mycobacterial infection, all seven patients with spinal TB and three of five patients with TB arthritis had positive ELISPOT assays. All nine patients with spinal TB had positive ELISPOT assays, but only four of six patients with TB arthritis had positive ELISPOT assays. The sensitivity, specificity, positive predictive value, and negative predictive value for skeletal TB diagnosis by the ELISPOT assay were 86.7%, 61.9%, 61.9%, and 86.7%, respectively. In conclusion, the ELISPOT assay can provide useful support in diagnosing skeletal TB, and spinal TB can be excluded based on a negative ELISPOT assay. ? 2011 Springer-Verlag. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/561119 | ISSN: | 0934-9723 | DOI: | 10.1007/s10096-011-1152-y | SDG/關鍵字: | gamma interferon; adult; article; bacterium culture; clinical article; controlled study; diagnostic accuracy; diagnostic test accuracy study; diagnostic value; enzyme linked immunospot assay; female; histopathology; human; human tissue; male; mycobacteriosis; predictive value; priority journal; sensitivity and specificity; Taiwan; tertiary health care; tuberculous arthritis; tuberculous osteomyelitis; tuberculous spondylitis; Adult; Aged; Clinical Laboratory Techniques; Enzyme-Linked Immunospot Assay; Female; Histocytochemistry; Hospitals; Humans; Interferon-gamma; Male; Middle Aged; Predictive Value of Tests; Sensitivity and Specificity; Taiwan; Tuberculosis, Osteoarticular |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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